ELISA METHOD TO DETECT ABO BLOOD GROUP IN EXTERNAL SECRETION FLUIDS

Acta Biochimica Indonesiana

View Publication Info
 
 
Field Value
 
Title ELISA METHOD TO DETECT ABO BLOOD GROUP IN EXTERNAL SECRETION FLUIDS
 
Creator Sadikin, Abdul Halim
Moenadjat, Yefta
Hardiany, Novi Sylvia
 
Description Background: Usually it takes a large number of volume sample to determine blood group from external secretion fluids. But, in certain condition, samples are only available in very small amount. The objective of this study is to detect the presence of ABO blood group substances in mucosal fluid using ELISA technique, thus only requires small amount of samples.Objective: To develop an ELISA technique using the current anti-ABO antibodies for determination of blood group by hemagglutination technique and second peroxidase label antibody specific for mouse IgG, originally used for another ELISA technique.Methods: 100 μl of diluted human intestinal mucosal fluid were incubated overnight in 4oC in ELISA microplate wells, followed by addition anti-ABO antibodies. Then after incubation, a second revealing antibody anti mouse IgG labeled with peroxidase was added. After a brief incubation, substrate H2O2 and chromogenic TMB were added.Results: Positive reaction is marked by development of blue colour, which, on termination enzymatic reaction by addition 100 μl H2SO4 change to yellow.Conclusion: An ELISA method for detecting ABO substance in mucosal fluid can be developed from antibodies not specifically made for this technique, but specific only for the target.
 
Publisher PP PBBMI
 
Contributor
 
Date 2019-09-21
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/33
10.32889/actabioina.v2i1.33
 
Source Acta Biochimica Indonesiana; Vol 2, No 1 (2019): ACTA BIOCHIMICA INDONESIANA; 31-38
2654-3222
2654-6108
 
Language eng
 
Relation https://pbbmi.org/jurnal/index.php/ActaBioIna/article/view/33/25
 
Rights Copyright (c) 2019 Abdul Halim Sadikin, Yefta Moenadjat, Novi Sylvia Hardiany
https://creativecommons.org/licenses/by-nc/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library