POLLEN STORAGE AND VIABILITY OF TWELVE CITRUS GENOTYPES IN BALI, INDONESIA

Scripta Biologica

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Field Value
 
Title POLLEN STORAGE AND VIABILITY OF TWELVE CITRUS GENOTYPES IN BALI, INDONESIA
 
Creator Astarini, Ida Ayu
 
Subject Biology
Plant breeding; horticulture; plant tissue culture
Plant Biology
 
Description The aim of this research was to obtain a method for pollen storage of Citrus species in Bali. Pollen from 12 Citrus genotypes was collected from five different regions; Petang, Plaga, Kintamani, Besakih, and Payangan Village.  Pollen from intact anthers were stored at three different storage temperatures, i.e., 25oC (room temperature), 4oC (refrigerator), and -20oC (freezer), with or without oven dried at 40oC for 20 hours before storage.  Pollen viability was determined using in vitro germination test.  Germination media contained 20 g/l sucrose and 8 g/l agar.  Result shows that -20oC was the best storage temperature.  At this temperature, only slight reduction of pollen viability after 2 months storage.  Storage at room temperature (25oC – 30oC) was not effective; pollen was infected with fungus within a week of storage. Oven dried pre-treatment before storage made better performance on pollen stored at 4oC.  Pollen moisture content decreased after oven dried, which also decreased fungus infection.  Storage of pollen is an important aspect to plant breeding programs, when individuals to be hybridized do not flower at the same time or are geographically separated.  This is the first report on pollen storage and viability of Citrus genotypes grown in Bali. 
 
Publisher Fakultas Biologi | Universitas Jenderal Soedirman
 
Contributor
 
Date 2019-06-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

Pollen viability test after storage

 
Identifier https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/765
 
Source Scripta Biologica; Articles in Press
Scripta Biologica; Articles in Press
2355-3138
 
Language en
 
Coverage Bali

pollen
 
Rights Copyright (c) 2019 Ida Ayu Astarini
http://creativecommons.org/licenses/by-sa/4.0
 

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