Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance

Acta Biológica Colombiana

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Title Identification of polymorphisms in the Cassava RXAM1 Gene and the association with Cassava Bacterial Blight Resistance

Identificación de polimorfismos en el gen RXAM1 de yuca y su asociación con la resistencia a la bacteriosis vascular
 
Creator Barrera, Andrea del Pilar
Soto-Sedano, Johana
López Carrascal, Camilo Ernesto
 
Subject Biología; fitopatologia; biologia molecular
Alelo; diversidad; receptor; síntomas de plantas
Biology; phytopathology; molecular biology
Allele; diversity; receptor; symptoms in plants


 
Description Cassava (Manihot esculenta Crantz) is an essential crop in tropical regions providing food for about one billion people around the world. The most important bacterial disease is cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam). Recently, a resistance gene called RXAM1 was identified, which codes for a protein that has an extracellular LRR (Leucine-Rich Repeat) domain and a cytoplasmic STK (Serine Threonine Kinase) domain. RXAM1 colocalizes with a QTL explaining 13 % of the resistance to the CIO136 strain of Xam. In this work, the response to infection with the strain XamCIO136 in ten different varieties of cassava was evaluated, which allowed the classification of the cassava varieties TMS60444, SG10735, MCOL1522, MCOL1505 and MCOL2215 as susceptible, while CM6438-14, CM523-7, and MBRA902 were cataloged as resistant. Also, SNPs (Single Nucleotide Polymorphism) polymorphisms were identified in the RXAM1 gene in the same group of cassava varieties. SG10735, CM6438-14, TMS6044, and MBRA685 presented the highest level of polymorphisms, while the varieties CM523-7, CM2177-2 and MCOL1522 were less polymorphic for this gene. The statistical analyses did not allow to identify a significant association between the phenotype and the identified polymorphisms. This study represents a first effort towards the establishment of associating between allelic variants and the cassava response to bacterial blight.

La yuca (Manihot esculenta Crantz) es un cultivo importante en regiones del trópico que proporciona alimento para cerca de 1000 millones de personas en todo el mundo. La enfermedad bacteriana más importante es la bacteriosis vascular causada por Xanthomonas axonopodis pv. manihotis (Xam). Recientemente se logró identificar un gen de resistencia denominado RXAM1, el cual codifica para una proteína que posee un dominio LRR (Leucine-Rich Repeat) extracelular y un dominio STK (Serine Threonine Kinase) citoplasmático. RXAM1 colocaliza con un QTL que explica el 13 % de la resistencia a la cepa CIO136 de Xam. En este trabajo se evaluó la respuesta a la infección con la cepa XamCIO136 en diez diferentes variedades de yuca lo cual permitió identificar que las variedades TMS60444, SG10735, MCOL1522, MCOL1505 y MCOL2215 fueron susceptibles, mientras que CM6438-14, CM523-7 y MBRA902 se comportaron como resistentes. Así mismo se identificaron polimorfismos tipo SNPs (Single Nucleotide Polymorphism) en el gen RXAM1 en el mismo grupo de variedades. Las variedades SG10735, CM6438-14, TMS6044 y MBRA685 presentaron el mayor nivel de polimorfismos, mientras que las variedades CM523-7, CM2177-2 y MCOL1522 fueron menos polimórficas para este gen. Los análisis estadísticos no permitieron identificar una asociación significativa entre el fenotipo y los polimorfismos identificados. Este estudio representa un primer esfuerzo con miras a asociar variantes alélicas con el fenotipo de respuesta a la bacteriosis vascular.
 
Publisher Universidad Nacional de Colombia - Sede Bogotá - Faculdad de Ciencias - Departamento de Biología
 
Contributor
 
Date 2020-05-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion


 
Format application/pdf
 
Identifier https://revistas.unal.edu.co/index.php/actabiol/article/view/77564
10.15446/abc.v25n2.77564
 
Source Acta Biológica Colombiana; Vol. 25, Núm. 2 (2020): En prensa / In press
Acta Biológica Colombiana; Vol. 25, Núm. 2 (2020): En prensa / In press
1900-1649
0120-548X
 
Language spa
 
Relation https://revistas.unal.edu.co/index.php/actabiol/article/view/77564/pdf
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https://creativecommons.org/licenses/by-nc-sa/4.0
 

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