Effect of Bacillus sp. and Trichoderma sp. in Micelial Growth of Sclerotium rolfsii

Acta Brasiliensis

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Title Effect of Bacillus sp. and Trichoderma sp. in Micelial Growth of Sclerotium rolfsii

Efeito de Bacillus sp. e Trichoderma sp. no crescimento micelial de Sclerotium rolfsii
 
Creator Ferreira de Sá, Mylenna Nádja
Lima, Jéssica de Souza
de Jesus, Fábio Nascimento
Perez, Jane Oliveira
Gava, Carlos Alberto Tuão
 
Description This research aimed to evaluate the antagonistic effect of bacterial isolates of the genus Bacillus sp. and the fungus Trichoderma sp. on the mycelial growth of Sclerotium rolfsii, causal agent of sclerotia rot in plants Vigna unguiculata (L.) Walp. (cowpea). The assay was conducted using the crop pairing technique. The pathogen was obtained by the isolation of V. unguiculata (L.) Walp. with symptoms of the disease. The antagonists used were: Bacillus subtilis (LCB 30), B. subtilis (LCB 45), Bacillus sp. (BMH), Bacillus sp. (INV) and Trichoderma sp. Evaluations of mycelial growth were made when the entire surface of the culture medium was presented BDA-colonized by S. rolfsii in the treatment control (pathogen grown in the absence of antagonist). For the analyzes, a completely randomized design with five replications was used. The isolates B. subtilis (LCB30), Bacillus sp. (INV) and Trichoderma sp. They are capable of inhibiting fungus mycelial growth of S. rolfsii by reducing the fungal colony on 53.77%, 29.22% and 34.00%, respectively, with isolated B. subtilis (LCB45) and Bacillus sp. (BMH), inhibited 13.55% and 21.55% growth S.rolfsii, respectively, did not differ statistically from the control.
Esta pesquisa teve como objetivo, avaliar o efeito antagônico de isolados bacterianos do gênero Bacillus sp. e do fungo Trichoderma sp. sobre o crescimento micelial de Sclerotium rolfsii, agente causador da podridão de esclerócio em plantas de feijão caupi (Vigna unguiculata (L.) Walp). O ensaio foi conduzido utilizando a técnica de pareamento de culturas. O patógeno foi obtido pelo isolamento de plantas de feijão-caupi com sintomas da doença. Os antagonistas utilizados foram: Bacillus subtilis (LCB 30), B. subtilis (LCB 45), Bacillus sp. (BMH), Bacillus sp. (INV) e Trichoderma sp. As avaliações do crescimento micelial foram realizadas quando toda a superfície do meio de cultivo BDA (Batata, Dextrose e Ágar) apresentava-se colonizada pelo S. rolfsii no tratamento testemunha (patógeno cultivado na ausência do antagonista). Para as análises foi utilizado o delineamento inteiramente casualizado, com cinco repetições. Os isolados B. subtilis (LCB30), Bacillus sp. (INV) e Trichoderma sp. são capazes de inibir o crescimento micelial do fungo S. rolfsii, reduzindo a colônia fúngica em 53,77%, 29,22% e 34,00%, respectivamente, sendo que os isolados B. subtilis (LCB45) e Bacillus sp. (BMH), inibiram 13,55% e 21,55% do crescimento de S. rolfsii, respectivamente, não diferindo estatisticamente da testemunha.
 
Publisher Universidade Federal de Campina Grande - UFCG
 
Date 2019-05-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/158
10.22571/2526-4338158
 
Source Acta Brasiliensis; Vol 3 No 2 (2019): Acta Brasiliensis; 79-81
Acta Brasiliensis; Vol 3 No 2 (2019): Acta Brasiliensis; 79-81
Acta Brasiliensis; Vol 3 No 2 (2019): Acta Brasiliensis; 79-81
2526-4338
2526-432X
 
Language por
 
Relation http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/158/68
 

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