Anti-inflammatory Activity of Crude Fucoidan from Sargassum crassifolium through Inhibition of ICAM-1 and VCAM-1 in Lipopolysaccharide-Induced Raw 264.7 Cell

Jurnal Ilmu Kefarmasian Indonesia

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Title Anti-inflammatory Activity of Crude Fucoidan from Sargassum crassifolium through Inhibition of ICAM-1 and VCAM-1 in Lipopolysaccharide-Induced Raw 264.7 Cell
Pengaruh Crude Fukoidan dari Ekstrak Sargassum crassifolium terhadap Kadar ICAM-1 dan VCAM-1 pada Sel Raw 264.7 yang Diinduksi Lipopolisakarida
 
Creator Werawati, Ayu
Kusmardi, Kusmardi
 
Description Inflammation is the response of vascular tissue to infection and damaged tissue to remove agents that cause inflammation. Macrophages are the dominant cells in the inflammatory reaction. Adhesion of leukocytes to vascular endothelium is a main feature of the inflammatory process. The presence of adhesion molecules, such as ICAM-1 and VCAM-1, can lead to adhesion of monocytes and lymphocytes to endothelial cells via bonding adhesion molecules to endothelial cells. Crude fukoidan derived from Sargassum crassifolium taken from Garut waters and extracted with dilute HCl, tested for anti-inflammatory activity through inhibition of ICAM-1 and VCAM-1 using  lipopolysaccharide-induced RAW 264.7 cell models. The viability test was carried out in the concentration range of 0.49-1000 µg / mL. The pattern of inhibition of cell proliferation is influenced by concentration. The highest concentration that caused cell viability of 80% was the concentration of 86.46μg / ml. Crude fukoidan reduced ICAM-1 and VCAM-1 levels at concentrations of 50 and 25 μg / ml.. Percent inhibition of ICAM-1 at 50 µg / mL concentration  was 61.04% compared to control and 74.26% at 25 µg / mL concentration. While the percent inhibition of VCAM-1 a
Inflamasi adalah respon jaringan pembuluh darah terhadap infeksi dan jaringan yang rusak untuk menghilangkan penyebab inflamasi. Tapi dalam beberapa kondisi, inflamasi dapat menyebabkan timbulnya penyakit kronis, seperti aterosklerosis.  Adesi leukosit ke endotelium vaskular merupakan ciri proses inflamasi Adanya molekul adesi, seperti ICAM-1dan VCAM-1 yang meningkat dapat menyebabkan adesi monosit dan limfosit pada sel endotel melalui ikatan molekul adesi pada sel endotel.  Crude fukoidan berasal dari Sargassum crassifolium yang diambil dari perairan Garut dan diekstraksi dengan HCl encer, diuji aktivitas antiinflamasi melalui penghambatan kadar ICAM-1 dan VCAM-1 menggunakan model sel RAW 264.7 yang diinduksi inflamasi dengan lipopolisakarida. Uji viabilitas dilakukan  dengan metode MTT pada rentang konsentrasi 0,49-1000 µg/mL. Pola penghambatan proliferasi sel dipengaruhi oleh konsentrasi. Dari uji viabilitas didapat konsentrasi tertinggi yang menyebabkan viabilitas sel sebesar 80% adalah konsentrasi crude fukoidan 86,46 μg/ml. Crude fukoidan terbukti dapat menurunkan kadar ICAM-1 dan VCAM-1  pada konsentrasi 50 dan 25 μg/ml. Persen penghambatan kadar ICAM pada konsentrasi 50 µg/mL adalah 61,04% dibanding kontrol inflamasi dan 74,26% pada konsentrasi 25 µg/mL.  Sementara persen penghamabatan kadar VCAM-1 pada konsentrasi 50 µg/mL adalah74,76% dibanding kontrol inflamasi dan 79,94% pada konsentrasi 25 µg/mL.
 
Publisher Fakultas Farmasi Universitas Indonesia
 
Date 2019-04-24
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/704
10.35814/jifi.v17i1.704
 
Source JURNAL ILMU KEFARMASIAN INDONESIA; Vol 17 No 1 (2019): JIFI; 95-102
2614-6495
1693-1831
 
Language eng
 
Relation http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/704/486
 
Rights Copyright (c) 2019 JURNAL ILMU KEFARMASIAN INDONESIA
 

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