Activity of Compounds in Chocolate Seaweed as Anti-atherosclerosis with Ligand Comparison HMG-COA Reductase-simvastatin and in-Silico Toxicity Test

Jurnal Ilmu Kefarmasian Indonesia

View Publication Info
 
 
Field Value
 
Title Activity of Compounds in Chocolate Seaweed as Anti-atherosclerosis with Ligand Comparison HMG-COA Reductase-simvastatin and in-Silico Toxicity Test
Anti-aterosklerosis Senyawa Sargassum Sp. pada HMG-COA Reductase (1HW9) dan Toksisitasnya secara in-Silico
 
Creator Zaidan, Sarah
Rahmat, Deni
Djamil, Ratna
 
Description Introduction:Sargassum.sp is one of the marine biota published as antiaterosclerosis, but compound toxicity data need to be explored for safety.
Method: Virtual screening has been done with MVD software from active compounds contained in sargassum where has activity as antiaterosclerosis with the mechanism of hypolipidemic effects. Test compounds in sargassum include: fucoidan, rhamnose, fucose, galactose, fucoxantin, alginate, phlorofucofuroeckol A, phloroglucinol, phlorotannin, with HMG-COA Reductase-Simvastatin adenosine receptors with a 1HW9 /PDB code. Its toxicity is predicted using pkCSM (online).
Results: The value RS, RMSD value as a result of the docking simulation carried out on said compounds, the following results are obtained: fucoidan (-110,420; 1,478), rhamnose (-72,081; 1,629), fucose (-98,408; 1,546), galactose (-95,757; 5,187), fucoxantin (-106,297; 2,161), alginate (-84,674; 2,897), phlorofucofuroeckol A (-106.701; 2,809), phloroglucinol (-103,140; 2,142), phlorotannin (-48,826; 7,750). The prediction results of toxicity showed fucoidan, rhamnose, fucose, galactose, fucoxantin, alginate, phlorofucofuroeckol A, phloroglucinol and phlorotannin not toxic with LD50 0.95-2.482g / kg.
Conclusion:Based on RS values, fucoidan, fucoxantin and phlorofucofuroeckol A compounds contained in brown seaweed were predicted to have activity as antiaterosclerosis. Compounds in brown seaweed can also be predicted to be relatively non-toxic with a value of LD50 0.95-2.482g / kg.
Latar Belakang: Rumput laut cokelat (Sargassum.sp) salah satu biota laut yang senyawanya telah dipublikasi mempunyai khasiat sebagai antiaterosklerosis, namun data toksisitasnya perlu juga dieksplorasi keamanannya.
Tujuan: Peneltian ini bertujuan untuk mendapatkan senyawa aktif  dari sargassum sp. dan toksisitasnya secara in-silico.
Metode:Penapisan secara virtual dengan perangkat lunak Molegro Virtual Docker (MVD) dari senyawa-senyawa aktif yang terkandung di sargassum dimana diduga mempunyai aktivitas antiaterosklerosis dengan mekanisme efek hipolipidemik. Senyawa uji dalam sargassum antara lain: fucoidan, rhamnose, fucose, galactose, fucoxantin, alginate, phlorofucofuroeckol A, phloroglucinol, phlorotannin, dengan reseptor adenosine HMG-COA Reductase-Simvastatin dengan kode PDB 1HW9. Toksisitasnya diprediksi menggunakan pkCSM (online).
Hasil:Nilai RS;nilai RMSD sebagai hasil dari simulasi docking yang dilakukan pada  senyawa-senyawa terssebut, didapat   hasil   sebagai berikut: fucoidan(-110.420;1.478),     rhamnose   (-72.081;1.629), fucose (-98.408;1.546), galactose (-95.757;5.187), fucoxantin (-106.297;2.161),alginate (-84.674;2.897), phlorofucofuroeckol A (-106.701;2.809), phloroglucinol (-103.140;2.142),phlorotannin(-48.826;7.750). Hasil prediksi toksisitas menunjukkan fucoidan, rhamnose, fucose, galactose, fucoxantin, alginate, phlorofucofuroeckol A, phloroglucinol dan phlorotannin tidak bersifat toksik dengan LD50 0.95-2.482g/kg.
Kesimpulan:Berdasarkan nilai RS didapatkan senyawa fucoidan, fucoxantin dan phlorofucofuroeckol A yang terkandung dalam rumput laut cokelat diprediksi mempunyai aktivitas sebagai antiaterosklerosis. Senyawa dalam rumput laut cokelat juga dapat diprediksi relatif tidak toksik dengan nilai LD50 0.95-2.482g/kg.
 
Publisher Fakultas Farmasi Universitas Indonesia
 
Date 2019-04-24
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/557
10.35814/jifi.v17i1.557
 
Source JURNAL ILMU KEFARMASIAN INDONESIA; Vol 17 No 1 (2019): JIFI; 120-125
2614-6495
1693-1831
 
Language eng
 
Relation http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/557/490
 
Rights Copyright (c) 2019 JURNAL ILMU KEFARMASIAN INDONESIA
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library