In Vitro Enzyme Tyrosinase Inhibitory Activity Test on Liquorice Root Extract Cream (Glycyrrhiza glabra L.)

Jurnal Ilmu Kefarmasian Indonesia

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Title In Vitro Enzyme Tyrosinase Inhibitory Activity Test on Liquorice Root Extract Cream (Glycyrrhiza glabra L.)
Uji Aktivitas Inhibisi Enzim Tirosinase In-vitro Krim Ekstrak Akar Manis (Glycyrrhiza glabra L.)
Creator Noor, Siti Umroh
Faridah, Faridah
Magdalena, Pamela
Description Liquorice root extract (Glycyrrhiza glabra L.) contains glabridin, an isoflavone substance that has the ability to inhibit the oxidation of L-tyrosin and L-DOPA in the formation of melanin, therefore it can be used as skin whitening. The aim of the study was to determine the potential of the best skin whitening cream using positive control of kojic acid. Formulated 1 blank formula, 3 cream formulas using various concentrations of ethanol extract 70% liquorice roots of (0.01, 0.11, 1.01)% based on the value of tyrosinase inhibition activity in vitro from extracts of (50, 75, 100)%. Spectrophotometric method is used to measure the absorption of dopacrome by a microplate reader which was incubated at 37°C at λ 490 nm for 20 minutes. Oil in water cream was prepared by mixing extract with cream base at a temperature of 70-75°C at a speed of 400 rpm for 25 minutes. Stability test was carried out for 4 weeks at room temperature storage and a temperature of 40°C, evaluated for physical quality. The results of determination of IC50 of kojic acid was 20.88 µg / mL; IC50 of extract variation were (126.75; 1130.90; and 10092.41) µg / mL respectively. Cream has milky white-yellowish color; smell of flowers; soft texture; homogeneous; there is no separation; has type M/A; plastic thixotropic flow properties; has an increasing value including viscosity, spreadability, globule size, and pH, which are respectively (455000-620000)cPs, (2695.82-3545.83)mm2, (54.66-66.27)μm , and (4.44-5.04); tyrosinase inhibition activity in formula 1, 2 and 3 were stored at weeks 0, 2nd  and 4th  were respectively (1.78; -25.74; 22.04)%, (6.74; 6, 12; 4.49)%, and (-28.78; 53.06; 20.32)%. It can be concluded that the cream formula containing liquorice root extract with a concentration of 1.01% is the best formula so it can be used as skin whitening.
Ekstrak akar manis (Glycyrrhiza glabra L.) mengandung senyawa isoflavon glabridin yang dapat menghambat oksidasi l-tirosin dan L-DOPA dalam pembentukan melanin sehingga berkhasiat sebagai pemutih kulit. Tujuan penelitian menentukan potensi terbaik krim pemutih kulit menggunakan kontrol positif asam kojat. Diformulasi 1 formula blangko, 3 formula krim menggunakan variasi konsentrasi ekstrak etanol 70% akar manis (0,01, 0,11, 1,01)% berdasarkan konsentrasi inhibisi tirosinase in vitro ekstrak (IC50, 75, 100)%. Digunakan metode secara spektrofotometri dengan mengukur serapan dopakrom menggunakan microplate reader diinkubasi suhu 37°C, λ 490 nm, 20 menit. Krim M/A dibuat dengan mencampurkan ekstrak bersama basis bersuhu 70-75°C, 400 rpm, 25 menit. Uji stabilitas dilakukan 4 minggu pada penyimpanan suhu kamar dan 40°C, dievaluasi mutu fisiknya. Hasil IC50 asam kojat sebesar 20,88 µg/mL; IC50 variasi ekstrak berturut-turut sebesar (126,75; 1130,90; 10092,41) µg/mL. Krim berwarna putih susu-putih kekuningan; ber-aroma bunga; lembut; homogen; tidak terjadi pemisahan; bertipe M/A; sifat alir tiksotropik plastis; viskositas, kemampuan sebar, ukuran globul, pH semakin meningkat berturut-turut sebesar  (455000- 620000)cPs, (2695,82-3545,83)mm2, (54,66-66,27)μm, (4,44-5,04); aktivitas inhibisi tirosinase formula 1, 2 dan 3 penyimpanan minggu 0, 2 dan 4 berturut-turut sebesar (1,78;-25,74;22,04)%, (6,74;6,12;4,49)%, (-28,78;53,06;20,32)%. Dapat disimpulkan formula krim dengan konsentrasi ekstrak akar manis 1,01% merupakan formula terbaik sehingga dapat digunakan sebagai pemutih kulit.
Publisher Fakultas Farmasi Universitas Pancasila
Date 2018-10-30
Type info:eu-repo/semantics/article
Peer-reviewed Article
Format application/pdf
Source JURNAL ILMU KEFARMASIAN INDONESIA; Vol 16 No 2 (2018): JIFI; 150-158
Language eng

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