Identification of α-glucosidase Enzyme Inhibitor Compound from Ethanol 96% Extract of Yakon Leaves (Smallanthus sonchifolius [Poepp.& Endl.] H. Robinson)

Jurnal Ilmu Kefarmasian Indonesia

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Title Identification of α-glucosidase Enzyme Inhibitor Compound from Ethanol 96% Extract of Yakon Leaves (Smallanthus sonchifolius [Poepp.& Endl.] H. Robinson)
Identifikasi Senyawa Penghambat Enzim α-Glukosidase dari Ekstrak Etanol 96% Daun Yakon (Smallanthus sonchifolius [Poepp.& Endl.]H. Robinson)
 
Creator Aziz, Zuhelmi
Al Qisthi, Farhana Hanida
Yuliana, Nancy Dewi
Simanjuntak, Partomuan
 
Description The leaves of yacon (Smallanthussonchi folius [Poepp. &Endl.] H. Robinson) or in Indonesia known as “Daun insulin” (Insulin leaf), scientifically has various medicinal properties and one of them as antidiabetic. The aim of this research is to know α-glucosidase enzyme inhibitor compound in the ethanol 96% extract of yacon leaves. Fractionation of ethanol extract subjected to column chromatography (SiO2; dichloromethane-methanol-water = 5 : 5 : 1) gave 5 fractions (Fr -1 ~Fr – 5). The results of the -glucosidase enzyme inhibition activity test in vitro against the five fractions obtained the 3rd fraction as the most active of 87.84 %. Purification of the 3rd fraction with preparative thin layer chromatography (SiO2; n-BuOH-ethanol-water = 4 : 1 : 2.2) gave pure isolate as Fr 3-1. Identification of pure isolates Fr 3-1 was carried out by interpreted spectra of Ultraviolet, Fourier Transform-Infra Red, Mass Spectra (from LC-MS) and compared data on the chemical shift of the Nuclear Magnetic Resonance (proton and carbon) and determined as Nystose (GF3) compound. The results of the -glucosidase enzyme inhibition activity showed that      Fr 3-1 has IC50 of 21.36 ppm
Daun yakon (Smallanthus sonchifolius [Poepp.& Endl.] H. Robinson) atau di Indonesia lebih dikenal sebagai daun insulin, secara ilmiah memiliki berbagai khasiat dan salah satunya sebagai antidiabetes. Tujuan penelitian ini adalah untuk mengetahui senyawa kimia penghambat enzim α-glukosidase dari ekstrak etanol 96% daun yakon. Isolasi, dan pemurnian dilakukan dengan cara fraksinasi ekstrak etanol 96% daun yakon dilakukan dengan kromatografi kolom (SiO2; diklorometan-metanol-air = 5:5:1) menghasilkan 5 fraksi (Fr-1 ~ Fr-5). Hasil uji aktivitas penghambatan enzim α-glukosidase secara in vitro terhadap kelima fraksi diperoleh fraksi 3 (Fr-3) sebagai fraksi teraktif dengan persentase inhibisi sebesar 87,84 %.  Pemurnian fraksi Fr-3 dengan KLT preparatif (SiO2; n-BuOH-etanol-air = 4 : 1 : 2,2) menghasilkan isolat murni pada Fr 3-1. Identifikasi isolat murni Fr 3-1 pretasi data spektra Ultraviolet, Fourier Transform-Infra Red, Liquid Chromatography-Mass Spectrometry, dan membandingkan data pergeseran kimia Resonansi Magnet Inti (proton dan karbon) dan ditentukan sebagai senyawa kimia nystose (GF3). Hasil uji aktivitas penghambatan enzim α-glukosidase untuk Fr-3-1 adalah dengan IC50 sebesar 59,48 bpj.  
 
Publisher Fakultas Farmasi Universitas Indonesia
 
Date 2019-04-24
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/652
10.35814/jifi.v17i1.652
 
Source JURNAL ILMU KEFARMASIAN INDONESIA; Vol 17 No 1 (2019): JIFI; 21-26
2614-6495
1693-1831
 
Language eng
 
Relation http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/652/474
 
Rights Copyright (c) 2019 JURNAL ILMU KEFARMASIAN INDONESIA
 

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