IDENTIFIKASI FRAGMEN HV1 DNA MITOKONDRIA INDIVIDU DATARAN RENDAH DAN DATARAN TINGGI

Biosfer: Jurnal Tadris Biologi

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Field Value
 
Title
IDENTIFIKASI FRAGMEN HV1 DNA MITOKONDRIA INDIVIDU DATARAN RENDAH DAN DATARAN TINGGI
 
Creator Satiyarti, Rina Budi
Aminah, Siti
Rosahdi, Tina Dewi
 
Subject
mtDNA, HV1, PCR, lowland, highland, D-loop
 
Description
An individual adaptation towards the geographical altitude associated with genetic factors, one of the genetic materials of the most widely used to study the characteristic of individuals in the population is the mitochondrial DNA.Based on that condition, this research will deciding human Fragmen HVI mtDNA from Indramayu as lowland individual and Sukabumi as highland individual. The purpose of this research is to identify hair root and the epithelial cell of the mouth as a source DNA mitochondrial that can used for a amplification of human fragment DNA mitokondria template and to identify fragment territory HV1 mtDNA by using Polimerase Chain Reaction (PCR) technic and detecting the yield of PCR from lowland and highland individual. The steps include the lysis to do the sample of the hair root and the epithelial cell of the mouth, amplified HV1 fragment mtDNA to utilize Polimerase Chain Reaction (PCR) technique and the result of PCR with elektroforesis gel agarosa. This research has been successfully, hair root and the epithelial cell can do analyze DNA mitochondrial marked by the fragment measuring 0,4 kb in the area D-Loop mtDNA and it can easier amplification is hair root characterized by the presence of DNA bands in agarose gel electrophoresis.
 
Publisher UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG
 
Contributor

 
Date 2018-02-18
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://ejournal.radenintan.ac.id/index.php/biosfer/article/view/2296
10.24042/biosf.v8i2.2296
 
Source Biosfer: Jurnal Tadris Biologi; Vol 8, No 2 (2017): Biosfer: Jurnal Tadris Biologi; 15-27
Biosfer: Jurnal Tadris Biologi; Vol 8, No 2 (2017): Biosfer: Jurnal Tadris Biologi; 15-27
2580-4960
2086-5945
 
Language ind
 
Relation http://ejournal.radenintan.ac.id/index.php/biosfer/article/view/2296/1713
 
Rights Copyright (c) 2018 Biosfer : Jurnal Tadris Biologi
 

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