Ekspresi Interleukin-35 pada penderita karsinoma nasofaring undifferentiated stadium lanjut

Oto Rhino Laryngologica Indonesiana

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Title Ekspresi Interleukin-35 pada penderita karsinoma nasofaring undifferentiated stadium lanjut
 
Creator Prabowo, Imam
Nurhikmah, Siti
Setiamika, Made
Budiani, Dyah
 
Description Latar belakang: Karsinoma nasofaring (KNF) merupakan keganasan pada kepala leher yang berasal dari epitel nasofaring. Sel pada tumor ini dapat memproduksi interleukin-35 (IL-35) yang mendukung pertumbuhan tumor, angiogenesis, dan stimulus radikal bebas melalui jalur mieloid dendritic supressor cells (MDSC) serta menghambat respons imun. Tujuan: Penelitian bertujuan untuk menganalisis ekspresi IL-35 pada penderita karsinoma nasofaring undifferentiated pada stadium lanjut tanpa metastasis. Metode: Penelitian ini merupakan penelitian observasional analitik dengan consecutive sampling pada 30 sampel jaringan biopsi penderita KNF undifferentiated yang dilakukan pemeriksaan imunohistokimia dengan menggunakan antibodi monoclonal rabbit anti IL-35 (RabMAbs) untuk mengetahui ekspresi IL- 35 kemudian dilakukan penghitungan dengan Intensity Distribution Score (IDS). Hasil: Pengukuran IDS ekspresi IL-35 dari 30 sampel penderita didapatkan sebagian besar kelompok menunjukan hasil positif kuat dan sangat kuat sebanyak 27 penderita, 2 penderita positif lemah, dan 1 penderita negatif dengan rerata (mean) serta simpang baku (SD) 224,67±78,00. Kesimpulan: Terdapat peningkatan (overexpression) ekspresi IL-35 pada KNF undifferentiated stadium lanjut.Kata kunci: KNF undifferentiated stadium lanjut, interleukin-35, faktor prediktifABSTRACT Background: Nasopharyngeal carcinoma (NPC) is a head and neck malignancy originated from nasopharyngeal epithelium. The tumor cells produced interleukin-35 (IL-35), which support the growth of tumors, angiogenesis, and stimulation of free radical pathways through mieloid dendritic supressor cells (MDSC), and also inhibition of the immune response. Purpose: This study aimed to analyze the expression of IL-35 in the end state of undifferentiated nasopharyngeal carcinoma. Methods: Analytic observational study with consecutive sampling from 30 samples of biopsy tissue of undifferentiated NPC which underwent immunohistochemical detection with rabbit monoclonal antibody anti IL-35 (RabMAbs) to determine the expression of IL-35, and scoring by Intensity Distribution Score (IDS). Results: The expression IL-35 by IDS measurement from 30 samples resulted mostly as strong to very strong in 27 samples, weak positive in 2 samples and negative in 1 sample with mean and standard deviation 224.67±78.00. Conclusion: There was an overexpression of IL-35 in the end state of undifferentiated nasopharyngeal carcinoma.Keywords: Nasopharyngeal carcinoma undifferentiated, Interleukin-35, predictive factor
 
Publisher PERHATI-KL
 
Contributor
 
Date 2016-12-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://orli.or.id/index.php/orli/article/view/162
10.32637/orli.v46i2.162
 
Source Oto Rhino Laryngologica Indonesiana; Vol 46, No 2 (2016): Volume 46, No. 2 July - December 2016; 147-54
2598-3970
0216-3667
10.32637/orli.v46i2
 
Language eng
 
Relation http://orli.or.id/index.php/orli/article/view/162/142
 

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