A study of cytotoxicity and proliferation of Cosmos caudatus Kunth leaf extract in human gingival fibroblast culture

Dental Journal (Majalah Kedokteran Gigi)

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Field Value
 
Title A study of cytotoxicity and proliferation of Cosmos caudatus Kunth leaf extract in human gingival fibroblast culture
 
Creator Shabrina, Zhafira Nur
Sumarta, Ni Putu Mira
Pramono, Coen
 
Subject Cosmos caudatus leaf extract; wound healing; human gingival fibroblasts culture
 
Description Background: Post-extraction dental sockets clinically resolve within a period of 3-4 weeks. However, complete healing and bundling of gingival fibers may require several months. Medication is therefore required to accelerate the healing process. Cosmos caudatus (C. caudatus), a local plant with antioxidant properties and high calcium content, has the potential to promote wound healing while also reportedly capable of strengthening bone. Previous studies have demonstrated the effectiveness of C. caudatus as an alternative treatment for post-menopausal osteoporosis by investigating the dynamic and cellular parameters of bone histomorphometry. Purpose: The study aimed to examine the citotoxicity and proliferation of human gingival fibroblast cells culture after the application of C. caudatus extract. Methods: Cultures of human gingival fibroblast cells with 5x104 cell density were divided into two groups and placed in a 30-well culture dish. The control group contained human gingival fibroblast cell culture without extract, while the experimental group consisted of human gingival fibroblast cells culture with extract. The concentrations of extract were 1200 μg/ml, 600 μg/ml, 300 μg/ml, 150 μg/ml, and 75 μg/ml. A toxicity test was conducted and the optimum concentration evaluated using an MTT assay, while fibroblast numbers on were calculated days 1 and 2 by means of a hemocytometer. Research data was analyzed using a one-way ANOVA test. Results: No toxicity was found. The optimum concentration was 600 μg/ml and fibroblast proliferation was significantly higher in the experimental group compared to the control group, p=0.002 (P<0.05). Conclusion: C. caudatus leaf extract is non-toxic and increases the proliferation of human gingival fibroblast culture at an optimum concentration of 600 μg/ml.
 
Publisher Faculty of Dental Medicine, Universitas Airlangga
 
Contributor
 
Date 2018-12-31
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://e-journal.unair.ac.id/MKG/article/view/10366
10.20473/j.djmkg.v51.i4.p179-184
 
Source Dental Journal (Majalah Kedokteran Gigi); Vol 51, No 4 (2018): (December 2018) Article in press; 179-184
2442-9740
1978-3728
 
Language eng
 
Relation http://e-journal.unair.ac.id/MKG/article/view/10366/7165
 
Rights Copyright (c) 2018 Dental Journal (Majalah Kedokteran Gigi)
http://creativecommons.org/licenses/by-sa/4.0
 

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