Molecular survey on Merkel cell polyomavirus in patients with colorectal cancer

Medical Journal of Indonesia

View Publication Info
 
 
Field Value
 
Title Molecular survey on Merkel cell polyomavirus in patients with colorectal cancer
 
Creator Karbalaie Niya, Mohammad Hadi; Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran
Safarnezhad Tameshkel, Fahimeh; Gastrointestinal and Liver Disease Research Center, Iran University of Medical Sciences, Tehran
Alemrajabi, Mahdi; Firoozgar Clinical Research Development Center (FCRDC), Iran University of Medical Sciences, Tehran
Taherizadeh, Mahsa; Department of Virology, Pasteur Institute of Iran, Tehran
Keshavarz, Mohsen; Department of Virology, Iran University of Medical Sciences, Tehran
Rezaee, Mohsen; Iranian Biological Resource Center, Microorganism’s Bank, Tehran
Keyvani, Hossein; Department of Virology, Iran University of Medical Sciences, Tehran
 
Subject cancer; colorectal; Merkel cell polyomavirus; real-time PCR; viral infection
 
Description Background: Merkel cell polyomavirus (MCV) has been associated with Merkel cell carcinoma (MCC) in humans, and its role in other human cancers is under investigation. The aim of this study was to investigate MCV genome infection in patients with colorectal cancer (CRC).Methods: This retrospective, case-control study used archived formalin-fixed, paraffin-embedded (FFPE) tissue samples from colorectal cancer patients (cases) and matched healthy subjects (controls) diagnosed by an expert pathologist from hospitals affiliated with Iran University of Medical Sciences, Tehran, Iran from 2011 to 2016. After DNA extraction with a QIAamp® DNA FFPE Tissue Kit, real-time polymerase chain reaction (PCR) was used for diagnosis. A positive control was produced by cloning with the Generay Biotechnology system. SPSS v.22 was used for analysis of demographic variables.Results: There were 157 participants included in the study: 66 were cases and 91 were controls. Their mean ages (±SD) were 59.35±14.48 and 57.21±14.66, respectively. The proportion of males was 57.6% in the case group and 57.1% in the control group. None of the samples were positive for MCV expression by real-time PCR assay. Association was detected between males with CRC and tumor location in the rectum and between males with CRC and the mucinous tumor type.Conclusion: None of the tissues from the CRC or non-cancerous control groups were positive for MCV genome infection, although a low viral load, the sample type, or the method of use should not be neglected. Further studies are recommended to obtain more comprehensive results.
 
Publisher Faculty of Medicine Universitas Indonesia
 
Contributor Iran University of Medical Sciences
 
Date 2018-12-31
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://mji.ui.ac.id/journal/index.php/mji/article/view/2759
10.13181/mji.v27i4.2759
 
Source Medical Journal of Indonesia; Vol 27, No 4 (2018): December; 229-36
2252-8083
0853-1773
10.13181/mji.v27i4
 
Language eng
 
Relation http://mji.ui.ac.id/journal/index.php/mji/article/view/2759/1260
 
Rights Copyright (c) 2018 Mohammad Hadi Karbalaie Niya, Fahimeh Safarnezhad Tameshkel, Mahdi Alemrajabi, Mahsa Taherizadeh, Mohsen Keshavarz, Mohsen Rezaee, Hossein Keyvani
http://creativecommons.org/licenses/by-nc/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library