PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)

Jurnal Pemuliaan Tanaman Hutan

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Title PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis)
 
Creator Nurtjahjaningsih, ILG
Herawan, Toni
Rachma, Reza Permatasari
Rimbawanto, Anto
 
Subject Tissue culture; primers screening; polymorphic loci; somaclonal variation
 
Description This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers.
 
Publisher Center for Forest Biotechnology and Tree Improvement (CFBTI)
 
Contributor BBPPBPTH
 
Date 2018-12-31
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4758
10.20886/jpth.2018.12.2.127-134
 
Source Jurnal Pemuliaan Tanaman Hutan; Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan; 127-134
2527-8665
1693-7147
 
Language eng
 
Relation http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/4758/4618
http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/downloadSuppFile/4758/450
 
Rights Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan
https://creativecommons.org/licenses/by-nc-sa/4.0
 

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