IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA HASIL FRAKSINASI EKSTRAK Phaseolus vulgaris L. DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY (GC-MS)

Jurnal Penelitian Pendidikan IPA (JPPIPA)

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Title IDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA HASIL FRAKSINASI EKSTRAK Phaseolus vulgaris L. DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY (GC-MS)
 
Creator Khair, Khurriyatul
Andayani, Yayuk
Hakim, Aliefman
 
Description This research was aimed to identify some classes of secondary metabolite compound on the fractionation results of green bean extract (P. vulgaris L.) using Gas Chromatography-Mass Spectroscopy (GC-MS) method. Green bean was extracted by methanol solvent. Fractionation of methanol extract used Vacuum Liquid Chromatography (VLC) with some variation of eluents such as n-hexane 100%; n-hexane: ethyl acetate (9 : 1 to 1 : 9); and ethyl acetate 100%, produced 11 major fractions.  Based on Thin Layer Chromatography (TLC) analysis using DCM: MeOH (9.5: 0.5) as mobile phases, these 11 major fractions were classified based on their polarity such as non-polar, semi-polar and polar fraction. The results of identification by GC-MS spectrometer showed the presence of secondary metabolites such as monoterpenes in non-polar fraction; in semi-polar fraction was found terpenoids and steroids; in polar fraction was found monoterpenes, phenolic and steroid as the lowest percent area, that is less than 2%. Keyword: P.vulgaris L extract, fractionation, VLC, GC-MS
 
Publisher Universitas Mataram
 
Contributor
 
Date 2017-01-31
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://jppipa.unram.ac.id/index.php/jppipa/article/view/51
10.29303/jppipa.v3i1.51
 
Source Jurnal Penelitian Pendidikan IPA; Vol 3, No 1 (2017): January
2407-795X
2460-2582
10.29303/jppipa.v3i1
 
Language eng
 
Relation http://jppipa.unram.ac.id/index.php/jppipa/article/view/51/50
 
Rights Copyright (c) 2017 Jurnal Penelitian Pendidikan IPA
 

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