Efficiency of Crystal Violet Stain to Study Mitotic Figures in Oral Epithelial Dysplasia


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Title Efficiency of Crystal Violet Stain to Study Mitotic Figures in Oral Epithelial Dysplasia
Creator Sajjad, Aneequa
Sajjad, Syeda Zaira
Minhas, Sadia
Kashif, Muhammad
Samad, Afra
Ali Sajid, Kanwar
Hasan, Munazza
Qureshi, Ihtesham-ud-Din
Subject Crystal violet stain
Mitotic figures
Oral epithelial dysplasia
Oral cancer
Description AIM: To evaluate mitotic activity in the different grades of oral epithelial dysplasia using 1% crystal violet stain.
MATERIAL AND METHODS: A descriptive study was conducted in the Department of Histopathology of the Post Graduate Medical Institute, Lahore on a total of thirty-three cases of the Oral Epithelial Dysplasia (OED). Fresh, frozen paraffin-embedded archival tissue blocks were collected from Lahore General Hospital, Lahore & Oral & Maxillofacial Surgery Department of Nawaz Sharif Hospital, Yakki Gate, Lahore. The representative sections were taken and, after processing, mounted on glass slides and stained with H&E and crystal violet stains. The stained slides were then examined under an optical microscope. The efficacy of 1% crystal violet stain to identify mitotic figures in the different grades of oral epithelial dysplasia was assessed with the sample t-test. A difference of p < 0.05 was considered to be significant.
RESULTS: A comparison of the mitotic figure count in two categories in sections stained with both stains showed a statistically significant difference. An increase in the mean mitotic count was noted in the sections of OED stained with crystal violet in comparison to the sections of OED stained with H&E which was statistically significant (p = 0.00).
CONCLUSION: Counting of mitotic cell is the rapid and simplest way of evaluating the proliferative activity of cells. Crystal violet stain can be a rationalised step in the staining of mitotic figures compared to the usual H&E staining and can be employed as a selective stain during routine histopathological procedures.
Publisher Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia; ID Design 2012/DOOEL Skopje, Republic of Macedonia
Date 2019-05-22
Type info:eu-repo/semantics/article
Format application/pdf
Identifier https://www.id-press.eu/mjms/article/view/oamjms.2019.330
Source Open Access Macedonian Journal of Medical Sciences; Vol 7 No 10 (2019): May 31 (OAMJMS); 1576-1581
Language eng
Relation https://www.id-press.eu/mjms/article/view/oamjms.2019.330/3398
Rights Copyright (c) 2019 Aneequa Sajjad, Syeda Zaira Sajjad, Sadia Minhas, Muhammad Kashif, Afra Samad, Kanwar Ali Sajid, Munazza Hasan, Ihtesham-ud-Din Qureshi

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