The Analysis of the Relationship between RELA Gene Expression and MMP-13 Gene Expression in Synoviocyte Cells after Mesenchymal Stem Cell Wharton Jelly

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Title The Analysis of the Relationship between RELA Gene Expression and MMP-13 Gene Expression in Synoviocyte Cells after Mesenchymal Stem Cell Wharton Jelly
 
Creator Sofia, Vivi
Nasrul, Ellyza
Manjas, Menkher
Revilla, Gusti
 
Subject Matrix Metalloproteinase-13
RELA
Mesenchymal Stem Cell Wharton Jelly
Co-culture
 
Description BACKGROUND: Therapy that can cure osteoarthritis with satisfactory results has not been found to date. In the pathogenesis of osteoarthritis, the genes involved in cartilage degradation include the RELA gene which plays an important role in modulating the occurrence of cartilage damage, which involves activation of pro-inflammatory cytokines. One of the cytokines involved in the cartilage degradation process is Matrix Metalloproteinase (MMP) -13 which is also modulated by NFĸβ.
AIM: This study aims to look at the expression of the RELA gene and expression of the MMP-13 gene and analyse the relationship of RELA gene expression with MMP-13 gene expression after administration of Mesenchymal Stem Cell Wharton Jelly in synoviocytes in vitro.
MATERIAL AND METHODS: This research is pure experimental research. The samples used derived from synovial tissue in osteoarthritis patients who underwent surgery for Total Knee Replacement (TKR). This study was divided into 6 treatment groups with 4 replications. Group I was the synoviocyte OA cell control group which was incubated 24 hours, group II was control of synoviocyte OA cell which was incubated 48 hours, group III was a group of Mesenchymal Stem Cell Wharton Jelly (MSC-WJ) which was incubated 24 hours, group IV was a Mesenchymal Stem Cell Wharton Jelly (MSC-WJ) cell group incubated 48 hours, group V was the co-culture group of synoviocyte-MSC-WJ cells incubated 24 hours and group VI was the co-culture of synoviocyte-MSC-WJ cells which were incubated 48 hours. Observation of MMP-13 gene expression and RELA gene in each group was carried out using qPCR.
RESULT: The results showed that the analysis of the relationship between RELA gene expression and MMP-13 gene expression in osteoarthritis synoviocytes cells after Mesenchymal Stem Cell Wharton Jelly as big as (r = 0.662).
CONCLUSION: The conclusion of this study is there was a strong correlation between RELA gene expression and MMP-13 gene expression in osteoarthritis synoviocytes after Mesenchymal Stem Cell Wharton Jelly (r = 0.662).
 
Publisher Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia; ID Design 2012/DOOEL Skopje, Republic of Macedonia
 
Date 2019-02-27
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
 
Identifier https://www.id-press.eu/mjms/article/view/oamjms.2019.135
10.3889/oamjms.2019.135
 
Source Open Access Macedonian Journal of Medical Sciences; Vol 7 No 4 (2019): Feb 28 (OAMJMS); 543-548
1857-9655
 
Language eng
 
Relation https://www.id-press.eu/mjms/article/view/oamjms.2019.135/2990
https://www.id-press.eu/mjms/article/view/oamjms.2019.135/2981
 
Rights Copyright (c) 2019 Vivi Sofia, Ellyza Nasrul, Menkher Manjas, Gusti Revilla
https://creativecommons.org/licenses/by-nc/4.0
 

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