Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay

Indonesian Journal of Cancer Chemoprevention

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Title Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay
 
Creator Lestari, Beni
Novitasari, Dhania
Putri, Herwandhani
Haryanti, Sari
Sasmito, Ediati
Meiyanto, Edy
 
Description Exposure of genotoxic substances come from various sources such as food additives. The aim of this study is to evaluate the genotoxicity of food additives in CHO-K1 cells by micronucleus test flow cytometry. The food additives: sodium saccharine (SS), monosodium glutamate (MSG), and sodium benzoate (SB) were assessed by in vitro cytotoxicity and genotoxicity using Chinese Hamster Ovary-K1 (CHO-K1) cells. The cytotoxic effect of those compounds was evaluated by MTT Assay on CHO-K1 Cells. The genotoxic evaluation was observed by in vitro micronucleus test by flowcytometry with double staining method. The results showed that the three compounds did not perform cytotoxic effect, increased the frequency of micronucleus, and changed the cell cycle profiles. In general, these studies obtained that none of three food additives showed cytotoxic and genotoxic effect on CHO-K1 cells. Micronucleus test using flow cytometry is suitable for this purpose study.Key words : food additives, genotoxic, cytotoxic, micronucleus
 
Publisher Indonesian Society for Cancer Chemoprevention
 
Contributor
 
Date 2017-06-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier https://ijcc.chemoprev.org/index.php/ijcc/article/view/145
10.14499/indonesianjcanchemoprev8iss2pp74-80
 
Source Indonesian Journal of Cancer Chemoprevention; Vol 8, No 2 (2017); 74-80
2355-8989
2088-0197
 
Language eng
 
Relation https://ijcc.chemoprev.org/index.php/ijcc/article/view/145/125
 
Rights Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention
 

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