Doxorubicin Induces Lamellipodia Formation and Cell Migration

Indonesian Journal of Cancer Chemoprevention

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Field Value
 
Title Doxorubicin Induces Lamellipodia Formation and Cell Migration
 
Creator Amalina, Nur Dina
Nurhayati, Ika Putri
Meiyanto, Edy
 
Description Breast cancer is the main cause of cancer death among women, especially breast cancer metastasis. Metastasis process begins with the ability of cell cancer invasion. Doxorubicin, a antracycline chemotheraphy, is known to induce TGFβ1, thus promote invasion. The aim of this study is to optimize doxorubicin doses to induce lamellipodia formation in 4T1 and MCF-7/HER2 cells. Lamellipodia formation was observed by morphological changes using microscope inverted. The effect of doxorubicin on cell viability was analyzed using MTT assay. Rac1 expression after doxorubicin exposure was determined by western blotting. Lamellipodia formation was observed by morphological change of the cell at the dose 10, 25, 50 and 100 nM. Doxorubicin at the dose of 10 nM could induced lamellipodia formation without affect cell viability in both 4T1 and MCF-7/HER2 cells.  Doxorubicin induced cell cycle arrest at G2/M phase at all doses. Doxorubicin 10 nM also decrease Rac1 expression compared to control.Key words: Doxorubicin, lamellipodia, Rac1, migration.
 
Publisher Indonesian Society for Cancer Chemoprevention
 
Contributor
 
Date 2017-06-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier https://ijcc.chemoprev.org/index.php/ijcc/article/view/143
10.14499/indonesianjcanchemoprev8iss2pp61-67
 
Source Indonesian Journal of Cancer Chemoprevention; Vol 8, No 2 (2017); 61-67
2355-8989
2088-0197
 
Language eng
 
Relation https://ijcc.chemoprev.org/index.php/ijcc/article/view/143/123
 
Rights Copyright (c) 2017 Indonesian Journal of Cancer Chemoprevention
 

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