Ekspresi IFN-Γ oleh Sel T CD4+ dan CD8+ Setelah Stimulasi Antigen Fusi ESAT-6- CFP-10 pada Pasien Tuberkulosis Paru Aktif

Buletin Penelitian Kesehatan

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Title Ekspresi IFN-Γ oleh Sel T CD4+ dan CD8+ Setelah Stimulasi Antigen Fusi ESAT-6- CFP-10 pada Pasien Tuberkulosis Paru Aktif
 
Creator Wibowo, Rahmad Yudha; Airlangga University
 
Subject
 
Description AbstractTuberculosis (TB) remains a major global health problem. T-cells mediated immunity contribute to protection from Mycobacterium tuberculosis infection. CD4+ and CD8+ T-cells secreted interferon gamma (IFN-γ) to stimulate fusion of phagolyzosome and produce free radicals for killing mycobacteria. Early secretory antigenic target 6 kD(ESAT-6) and culture filtrate protein 10(CFP-10) are dominant M. tuberculosis antigens in inducing IFN-γ. This study compared CD4+ and CD8+T-cells expressing IFN-γ with and without ESAT-6- CFP-10 fusion antigen stimulation in active tuberculosis patients. Peripheral blood mononuclear cellsfrom active TB patients were cultured with and without ESAT-6-CFP-10 fusion antigen. CD4+ and CD8+ T-cells expressing IFN-γ were examined using flow cytometry.CD4+ T-cells expressing IFN-γ after antigen stimulation compare to controls(1.51%±1.7 vs 1.23%±0.86; p=0.08),and CD8+ T-cells (1.58%±0,99 vs 1.92%±1.13; p=0.86).CD4+ and CD8+T-cells expressing IFN-γ after antigen stimulation (1.51%±1.7 vs 1.58%±0,99; p=0.94).There were no significant differences of CD4+ and CD8+T-cells expressing IFN-γ after ESAT-6-CFP-10 fusion antigen stimulation in active pulmonary tuberculosis patients.Key word: CD4+, CD8+, IFN-γ, ESAT-6-CFP-10 fusion antigenAbstrakTuberkulosis masih menjadi masalah kesehatan di dunia. Imunitas yang diperantarai sel T berperan penting dalam perlindungan terhadap infeksi Mycobacterium tuberculosis. SelTCD4+ dan CD8+ mensekresi IFN-γ yang menstimulasi fusi fagolisosom dan pembentukan radikal bebas untuk menghancurkan M. tuberculosis. ESAT-6 dan CFP-10 merupakan antigen M. tuberculosis yang dominan menginduksi IFN-γ. Penelitian membandingkan selT CD4+ dan CD8+ yang mengekspresikan IFN-γ setelah stimulasi antigen fusi ESAT-6-CFP-10 pada TB aktif.Sampel PBMC pasien TB aktif dikultur dengan dan tanpa stimulasi antigen fusi ESAT-6-CFP-10. Persentase sel TCD4+ dan CD8+ yang mengekspresikan IFN-γ diperiksa dengan flow cytometry. Sel TCD4+ yang mengekspresikan IFN-γ setelah stimulasi antigen dibandingkan dengan kontrol (1,51%±1,7 vs 1,23%±0,86; p=0,08) dan sel TCD8+ yang mengekspresikan IFN-γ(1,58%±0,99 vs 1.92%±1.13; p=0,86). Tidak terdapat perbedaan yang signifikan dalam ekspresi IFN-γ antara sel T CD4+ dan sel T CD8+ setelah stimulasi antigen fusi ESAT6-CFP10 pada pasien TB paru aktif.Kata kunci: Sel T, CD4+, CD8+, IFN-γ, antigen fusi ESAT-6 dan CFP-10
 
Publisher Badan Penelitian dan Pengembangan Kesehatan
 
Contributor
 
Date 2017-12-30
 
Type
 
Format application/pdf
 
Identifier http://ejournal.litbang.kemkes.go.id/index.php/BPK/article/view/6874
10.22435/bpk.v45i4.6874.223-226
 
Source Buletin Penelitian Kesehatan; Vol 45, No 4 (2017); 223-226
 
Language id
 
Relation http://ejournal.litbang.kemkes.go.id/index.php/BPK/article/downloadSuppFile/6874/15810
 
Rights The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
 

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