Characterisation of Lactic Acid Bacteria from Dengke Naniura of Common Carp (Cyprinus carpio) with α-Glucosidase Inhibitory Activity

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Title Characterisation of Lactic Acid Bacteria from Dengke Naniura of Common Carp (Cyprinus carpio) with α-Glucosidase Inhibitory Activity
 
Creator Hutahaean, Andi Josep Nicolas
Silalahi, Jansen
Suryanto, Dwi
Satria, Denny
 
Subject lactic acid bacteria
traditional food products
dengke naniura
 
Description BACKGROUND: Fermented foods were favourable because of its properties in enhancing the shelf life, safety, function, sensory and nutrition. There are many fermented foods tested in vitro as an α-glucosidase enzyme inhibitor. Dengke naniura is one of Indonesia’s traditional food made using fermentation.
AIM: To identify lactic acid bacteria (LAB) strains in dengke naniura and its properties in inhibiting the α-glucosidase enzyme.
METHODS: The carp were sacrificed, and soaked with rough lemon for 6 hours then spices added to it for another 1 hour. Then the isolation of LAB conducted using a serial dilution of the samples. The selected isolates of the LAB were then characterised by its morphology under the microscope, gram staining, growth at 15°C and 45°C and biochemical identification. The isolates were then tested for its inhibiting properties against the α-glucosidase enzyme.
RESULTS: The isolates (DL-109 and DL-107) were a gram-positive, nonspore-forming and non-motile rod. The Physiological and biochemical properties of the isolates confirm its LAB properties. On the test against α-glucosidase enzyme activity inhibition, isolate DL-109 LAB (4) showed dominant activity with very low IC50 compared to Acarbose (IC50 = 128.06 ppm) and DL-107 (46.32 ppm) while at the lowest dosage of 25 µg/ml DL-109 showed activity as much as 54.76%.
CONCLUSION: These findings concluded that the isolates were LAB by its properties and can be used for lowering blood glucose in term of inhibition of the α-glucosidase enzyme.
 
Publisher Scientific Foundation SPIROSKI, Skopje, Republic of Macedonia
 
Date 2019-11-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
 
Identifier https://www.id-press.eu/mjms/article/view/oamjms.2019.506
10.3889/oamjms.2019.506
 
Source Open Access Macedonian Journal of Medical Sciences; Vol. 7 No. 22 (2019): Nov 30 (Herbal Medicine in Pharmaceutical and Clinical Sciences); 3794-3798
1857-9655
 
Language eng
 
Relation https://www.id-press.eu/mjms/article/view/oamjms.2019.506/4270
https://www.id-press.eu/mjms/article/view/oamjms.2019.506/4243
 
Rights Copyright (c) 2019 Andi Josep Nicolas Hutahaean, Jansen Silalahi, Dwi Suryanto, Denny Satria (Author)
http://creativecommons.org/licenses/by-nc/4.0
 

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