Prevalence of antibodies against different serovars of Leptospira interrogans in canines in Boyeros municipality, Havana, Cuba

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Title Prevalence of antibodies against different serovars of Leptospira interrogans in canines in Boyeros municipality, Havana, Cuba
Prevalencia de anticuerpos a diferentes serovares de Leptospira interrogans en caninos del municipio Boyeros, La Habana, Cuba
 
Creator Rojas-Hoyos, Nicolás Andrés
Márquez-Álvarez, Mario
Pino-Rodríguez, Duniel
González-Chávez, Mitchell Torres
Cuba-Romero, Yaritza
Gainza-Santos, Noemí
 
Subject Leptospira; seroprevalence; dogs; agglutination; zoonosis
Leptospira; seroprevalencia; perros; aglutinación; zoonosis
 
Description In order to determine the prevalence of antibodies against some Leptospira interrogans serovars, blood samples were taken from 160 dogs older than a year. This study was carried out in Boyeros municipality, Havana, Cuba. The samples collected were centrifuged at 1350 g for 10 minutes and the sera obtained were stored in vials at -20°C. For the laboratory evaluation, a slow plate agglutination test was used for the detection of specific antibodies against L. canicola, L. icterohaemorrhagiae, L. pomona, L. ballum, L. australis, and L. hebdomadis. The samples that reacted against six antigen units of at least one strain of Leptospira spp. evaluated, from the 1: 100 dilution, were positive. Seroprevalence was determined by a cross-sectional observational epidemiological study, using the population estimation data. A total seroprevalence value of 63,1 % (101/160) was obtained against one or more serovars, with L. icterohaemorrhagiae, being more frequent with 58,8 % (94/160), followed by L. canicola with 47,5 % (76/160) L. ballum 22,5 % (36/160), L. pomona 18,1 % (29/160), L. australis 10 % (16/160), and  L. hebdomadis 7,5 %  (12/160).  This  study  demonstrated  a  high  seroprevalence in the canine population studied, which indicated a previous exposure to the agent and its wide spread.
Para determinar la prevalencia de anticuerpos contra algunos serovares de Leptospira interrogans se tomaron muestras de sangre a 160 perros mayores de un año del municipio Boyeros, la Habana, Cuba. Las muestras colectadas se centrifugaron a 1350 g durante10 minutos y los sueros obtenidos se conservaron en viales a -20°C. Para la detección de anticuerpos específicos contra L. canicola, L. icterohaemorrhagiae, L. pomona, L. ballum, L. australis y L. hebdomadis se empleó una prueba de Microaglutinación Lenta en Placa. Se consideraron positivas las muestras que reaccionaron frente a seis unidades de antígeno, de al menos una de las cepas de Leptospira spp. evaluadas, a partir de la dilución 1:100. La seroprevalencia se determinó mediante un estudio epidemiológico observacional de tipo transversal, con el uso de datos de estimación de la población del municipio. Se obtuvo un valor de seroprevalencia total de 63,1 % (101/160) contra uno o más serovares; resultó más frecuente L. icterohaemorrhagiae con 58,8 % (94/160), seguido de L. canicola con 47,5 % (76/160), L. ballum 22,5 % (36/160), L. pomona 18,1 % (29/160), L. australis 10 % (16/160) y L. hebdomadis 7,5 % (12/160). Este estudio demostró una alta seroprevalencia en la población canina estudiada, lo que indica una exposición previa al agente y una amplia circulación de este.
 
Publisher Centro Nacional de Sanidad Agropecuaria
 
Contributor

 
Date 2017-06-17
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format text/html
application/pdf
application/epub+zip
 
Identifier http://revistas.censa.edu.cu/index.php/RSA/article/view/871
 
Source Revista de Salud Animal; Vol. 39, Núm. 1 (2017): enero-abril; 35-42
2224-4700
0253-570X
 
Language spa
 
Relation http://revistas.censa.edu.cu/index.php/RSA/article/view/871/852
http://revistas.censa.edu.cu/index.php/RSA/article/view/871/841
http://revistas.censa.edu.cu/index.php/RSA/article/view/871/864
 
Rights Copyright (c) 2017 Revista de Salud Animal
 

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