Efek Beauveria bassiana pada Anopheles maculatus Fase Aquatik di Laboratorium

Buletin Penelitian Kesehatan

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Field Value
 
Title Efek Beauveria bassiana pada Anopheles maculatus Fase Aquatik di Laboratorium
 
Creator Ikawati, Bina; Balai Litbang P2B2 Banjarnegara Jl. Selamanik No. 16 A Banjarnegara, 53415 Jawa Tengah, Indonesia
Marbawati, Dewi; Balai Litbang P2B2 Banjarnegara Jl. Selamanik No. 16 A Banjarnegara, 53415 Jawa Tengah, Indonesia
Wahyudi, Bondan Fajar; Balai Litbang P2B2 Banjarnegara Jl. Selamanik No. 16 A Banjarnegara, 53415 Jawa Tengah, Indonesia
 
Subject
 
Description AbstractBeauveria bassiana can be used both as for controlling agricultural insect and protecting health. Thisstudy aims to examine the effects of aquatic phase of B. bassiana on An. maculatus in the laboratory.Samples were eggs and larvae of An. maculatus reared from Banjarnegara colony. Eggs and instarlarvae II, III and IV and their control which consisted of 10 larvae/eggs were replicated six times andcontacted to B. bassiana spores for 15 minutes and then transferred to aquades to be maintained forobservation, in every 24 hours as long as 192 hours (8 days). Probit analysis found that applicationof B. bassiana caused damage of external coat of eggs and inhibited >60% of unhatched eggs. Lethaldosage was dosage spores of 1,713x107 (16 days), whereas the lethal dose required to make 50% ofunperached eggs is a dose of spore concentration of 1,361x107 (11.6 days). Higher concentrationswill be needed to know the faster effects B bassiana on An. maculatus larvae or eggs.Key words: Beauveria bassiana, Anopheles maculatus, aquatic phase, laboratory AbstrakBeauveria bassiana dapat digunakan baik sebagai pengendali serangga pertanian maupun kesehatan.Penelitian ini bertujuan mengkaji efek B. bassiana terhadap An. maculatus pada fase akuatik dilaboratorium. Sampel uji berupa telur dan larva An. maculatus dari koloni Banjarnegara. Telur danlarva instar II,III dan IV serta kontrol masing-masing sebanyak 10 ekor/butir dengan replikasi 6 kalidikontakkan dengan spora B. bassiana selama 15 menit dan selanjutnya dipindahkan ke aquadesuntuk dipelihara untuk dilakukan pengamatan, pengamatan dengan mikroskop compound terutamapada larva yang lemah/mati setiap 24 jam selama 192 jam (8 hari). Analisis probit membuktikanbahwa aplikasi B. bassiana pada telur menimbulkan efek kerusakan pada lapisan luar telur diketahuidari pengamatan dengan mikroskop serta mampu menghambat >60% telur tidak menetas. Simulasidengan análisis probit menunjukkan lethal dose yang dibutuhkan untuk membunuh 50% larva uji(instar II,III maupun IV) adalah dosis konsentrasi spora 1,713x107 (16 hari), sedangkan lethal doseyang dibutuhkan untuk membuat 50% telur tidak menetas adalah dosis konsentrasi spora 1,361x107(11,69 hari). Dibutuhkan konsentrasi yang lebih tinggi untuk mempercepat efek B. bassiana terhadaplarva maupun telur An. maculatus.Kata kunci : Beauveria bassiana, Anopheles maculatus, aquatik, laboratorium
 
Publisher Badan Penelitian dan Pengembangan Kesehatan
 
Contributor
 
Date 2017-07-18
 
Type
 
Format application/pdf
 
Identifier http://ejournal.litbang.kemkes.go.id/index.php/BPK/article/view/7346
10.22435/bpk.v45i2.7346.137-144
 
Source Buletin Penelitian Kesehatan; Vol 45, No 2 (2017); 137-144
Buletin Penelitian Kesehatan; Vol 45, No 2 (2017); 137-144
 
Language en
 
Rights The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
 

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