Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender

Tropical Animal Science Journal

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Title Maintaining the Quality of Kacang Buck Semen in Chilled Storage with the Addition of Green Tea Extract in Extender
 
Creator Susilowati, S.
Mustofa, I.
Wurlina, W.
Hernawati, T.
Oktanella, Y.
 
Subject DNA fragmentation
intact plasma membrane
malondialdehyde
sperm motility
poverty reduction
 
Description Kacang goats can be bred through artificial insemination techniques using liquid semen from a superior buck. The study was aimed to determine the quality of Kacang buck semen in chilled storage when green tea extract (GTE) was added to the semen extender. Specifically, 12 ejaculates from three Kacang bucks were diluted in skim milk–egg yolk extender containing 0 mg, 0.05, 0.10, and 0.15 mg of GTE/100 mL. The extended semen was stored at 5°C, and its quality was evaluated daily for 5 d. Comparatively, semen with 0.10 mg of GTE/100 mL had the best quality (p<0.05): it had the highest sperm viability, sperm progressive motility, and sperm intact plasma membrane values, and the lowest malondialdehyde levels and DNA fragmentation percentage. However, as the storage period increased, there was a decrease in sperm viability, sperm motility, and sperm intact plasma membrane values with an increase in malondialdehyde levels and DNA fragmentation (p<0.05). The sperm motility of semen with 0.10 mg of GTE/100 mL was maintained during 5 d of chilled storage to meet the qualification for artificial insemination. In contrast, the sperm motility of semen with 0.05 mg and 0.15 mg of GTE/100 mL was maintained for 4 d, whereas that of the control semen was maintained only for 3 d. Thus, the addition 0.10 mg of GTE/100 mL of skim milk-egg yolk extender seems to help maintain the quality of Kacang goat sperm in chilled storage.
 
Publisher Faculty of Animal Science, Bogor Agricultural University
 
Date 2021-11-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://journal.ipb.ac.id/index.php/tasj/article/view/32738
10.5398/tasj.2021.44.4.408
 
Source Tropical Animal Science Journal; Vol. 44 No. 4 (2021): Tropical Animal Science Journal; 408-414
2615-790X
2615-787X
10.5398/tasj.2021.44.4
 
Language eng
 
Relation http://journal.ipb.ac.id/index.php/tasj/article/view/32738/22486
 
Rights Copyright (c) 2021 Tropical Animal Science Journal
http://creativecommons.org/licenses/by-sa/4.0
 

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