DNA Binding Activity of Marine Shrimp LvProfilin

KMUTNB: International Journal of Applied Science and Technology

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Title DNA Binding Activity of Marine Shrimp LvProfilin
Creator Laoong-u-thai, Yanisa; Department of Chemical Engineering, Faculty of Engineering, Burapha University, Chonburi, Thailand
Wanna, Warapond; <p>Center for Genomics and Bioinformatics Research, Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla, Thailand</p>
Kaikaew, Autaipohn
Subject Profilin, Marine shrimp, Phylogenetic tree, Tissue distribution, SWISS-MODEL, DNA binding protein
Description Shrimp farming is an important business in Thailand and worldwide. The study of molecular biology and biochemical pathway of the key molecules controlling muscle growth is an essential to improve shrimp livestock. Profilin is a pivotal protein in muscle formation, especially actin protein. Its nuclear function has been reported in many species for gene regulation. Here in this work, we characterized the function of LvProfilin, a marine shrimp profilin from Litopenaeus vannamei, both in silico and in vitro. The phylogenetic tree of LvProfilin among organisms and its 3D protein structure showed that LvProfilin was highly conserved among shrimp and arthropods. The homology modeling of its 3D structure revealed 3 alpha-helices and 6 beta-strands similar to most eukaryotic profilins. To interpret its possible function, the gene expression of LvProfilin in various tissues was performed. We found that this gene was expressed in various tissues. This result may imply that LvProfilin could share a common function in all tissues. Nuclear activity has been a promising function of LvProfilin. We performed a DNA/RNA binding prediction analysis using DRNApred. The result indicated that Lysine-90 and Threonine-91 were the putative DNA-binding sites with the probability of 63.12% and 54.16%, respectively. Its binding activity was confirmed in vitro which bound stronger to single strand DNA than double strand DNA. To our best knowledge, this is the first report of DNA binding activity of profilin in invertebrates.
Publisher Academic Enhancement Department
Date 2021-10-07
Type info:eu-repo/semantics/article

Format application/pdf
Identifier http://ojs.kmutnb.ac.th/index.php/ijst/article/view/5522
Source Applied Science and Engineering Progress; Vol 15, No 3 (Jul.–Sep. 2022): In progress; Article 5522
Relation http://ojs.kmutnb.ac.th/index.php/ijst/article/view/5522/pdf_318

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