DIAGNOSIS OF ORF IN WEST AFRICAN DWARF GOATS IN UYO, AKWA IBOM STATE, NIGERIA

African Journal of Infectious Diseases (AJID)

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Title DIAGNOSIS OF ORF IN WEST AFRICAN DWARF GOATS IN UYO, AKWA IBOM STATE, NIGERIA
 
Creator Adedeji, Adeyinka Jeremy
Maurice, Nanven Abraham
Wungak, Yiltawe Simwal
Adole, Jolly Amoche
Chima, Nneka Chineze
Woma, Timothy Yusufu
Chukwuedo, Anthony Amechi
Shamaki, David
 
Subject Tradtional Medicine, Medicinal Plants
Orf, goat, PCR, Akwa Ibom, Nigeria
 
Description Background: Sixty (60) male West African Dwarf goats were reported with clinical signs of enlarged lymph nodes, scabs
on the mouth, nose and ears. Two of the goats died and post mortem examination reveals enlarged submandibular lymph
nodes and vesicular lesions on the tongue. Clinical diagnosis of Orf has been reported in Nigeria but this report is the
confirmatory diagnosis of Orf in a suspected outbreak in an experimental farm in Uyo, Akwa Ibom State, Nigeria using
molecular techniques.
Materials and Methods: Scabs, spleen and lymph node samples from goats suspected to have died from Orf were
collected, transported on ice to the laboratory and homogenized. The DNA was extracted using QIAmp DNA minikit
(Qiagen) according to the manufacturer’s instructions. Orf virus (ORFV) was amplified using published ORFV specific
primers by PCR.
Results: Morbidity and mortality were 100% and 3.3% respectively, while ORFV was detected by PCR. Diagnosis of Orf
was confirmed based on clinical signs of enlarged lymph nodes, scabs on the mouth, nose and ears, necropsy findings of
enlarged submandibular lymph nodes and vesicular lesions on the tongue and PCR results.
Conclusion: This may be the first report of molecular diagnosis of Orf in Nigeria. The 100% morbidity and 3.3% mortality
rate is higher than previously reported thus Orf is becoming of greater economic importance than previously thought. It is
therefore recommended that routine laboratory diagnosis of Orf be carried nationwide to determine the prevalence of Orf in
Nigeria.
 
Publisher African Traditional Medicine Supporters Initiative (ATHMSI), 7, Road 1, Otunmaiye Square
 
Contributor Staff of Viral Research division, National Veterinary Research Institute, Vom, Mr. Dyek Yohana and Mr. Maguda Adrian, who assisted with the sample processing. We also wish to acknowledge Drs. T.M. Joannis, Luka Pam and David Lazarus who assisted us with p
 
Date 2017-06-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://journals.sfu.ca/africanem/index.php/AJID/article/view/4795
10.21010/ajid.v11i2.12
 
Source African Journal of Infectious Diseases (AJID); Vol 11, No 2 (2017); 90-94
2505-0419
2006-0165
 
Language eng
 
Relation http://journals.sfu.ca/africanem/index.php/AJID/article/view/4795/2999
 
Coverage Africa


 
Rights Copyright (c) 2017 African Journal of Infectious Diseases (AJID)
 

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