A COMPARISON OF RAPID DIAGNOSTIC TESTING (BY PLASMODIUM LACTATE DEHYDROGENASE), AND QUANTITATIVE BUFFY COAT TECHNIQUE IN MALARIA DIAGNOSIS IN CHILDREN

African Journal of Infectious Diseases (AJID)

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Title A COMPARISON OF RAPID DIAGNOSTIC TESTING (BY PLASMODIUM LACTATE DEHYDROGENASE), AND QUANTITATIVE BUFFY COAT TECHNIQUE IN MALARIA DIAGNOSIS IN CHILDREN
 
Creator Ifeorah, Ifeanyi Kanayo
Brown, Biobele J
Sodeinde, Olugbemiro O
 
Subject Tradtional Medicine, Medicinal Plants
malaria, quantitative buffy coat, rapid diagnostic testing
 
Description Background: The World Health Organization (WHO) considers early and rapid diagnosis as one of the strategies to
control malaria. This study compared the performance of Quantitative Buffy Coat (QBC) test and the Plasmodium lactate
dehydrogenase (pLDH) rapid diagnostic test (RDT) with microscopy as the gold standard.
Materials and Methods: The study involved children ages 0-5 years who presented with a history of fever at the
University College Hospital, Ibadan, Nigeria. Blood was collected from each patient and used for RDT, QBC and Giemsastained
blood films for malaria parasites (MP). Results of QBC and RDT were compared with microscopy results for the
diagnosis of malaria.
Results: A total of 370 cases (194 boys and 176 girls) were studied giving a male: female ratio of 1.1:1. Of the 370 cases
tested using Giemsa-stained thick blood films for MP, 78 (21 %) were positive. For the QBC test, 78 (21%) of the cases
were positive with sensitivity, specificity, positive and negative predictive values of 70.5 %, 92.1%, 70.5 % and 92.1 %
respectively. Seventy-six (20%) of the cases were positive by RDT with sensitivity, specificity, positive and negative
predictive values of 84.2 %, 95.2 %, 82.1 %, and 95.9 % respectively. There was no significant difference in the sensitivity
of QBC compared with the RDT.
Conclusion: Both the QBC and the pfLDH (RDT) performed reasonably well in this study Malaria rapid diagnostic tests
are recommended in malaria endemic clinical settings to avoid unnecessary antimalarial treatment.
 
Publisher African Traditional Medicine Supporters Initiative (ATHMSI), 7, Road 1, Otunmaiye Square
 
Contributor Mr Afolabi (Laboratory Scientist) and the members of the malaria proteomics team for their assistance. I also wish to acknowledge Mrs Patience N Ogunjobi, and Mrs Victoria U Nwachukwu, both of the Department of Medical Microbiology and Parasitology of the
 
Date 2017-06-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://journals.sfu.ca/africanem/index.php/AJID/article/view/4789
10.21010/ajid.v11i2.5
 
Source African Journal of Infectious Diseases (AJID); Vol 11, No 2 (2017); 31-38
2505-0419
2006-0165
 
Language eng
 
Relation http://journals.sfu.ca/africanem/index.php/AJID/article/view/4789/2992
 
Coverage Africa


 
Rights Copyright (c) 2017 African Journal of Infectious Diseases (AJID)
 

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