MULTILOCUS SEQUENCE TYPING OF CARBAPENEM RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES FROM PATIENTS PRESENTING AT PORT ELIZABETH HOSPITALS, SOUTH AFRICA

African Journal of Infectious Diseases (AJID)

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Title MULTILOCUS SEQUENCE TYPING OF CARBAPENEM RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES FROM PATIENTS PRESENTING AT PORT ELIZABETH HOSPITALS, SOUTH AFRICA
 
Creator Annear, D.
Black, J.
Govender, S.
 
Subject Tradtional Medicine, Medicinal Plants
Pseudomonas aeruginosa, VIM-2 carbapenemase, multi-locus sequence typing
 
Description Background: Pseudomonas aeruginosa is an important nosocomial pathogen that exhibits multiple drug resistance
with increasing frequency, especially to carbapenems making patient treatment difficult. Carbapenem-resistance may
be caused by porin gene mutations, active drug efflux, and carbapenemase production. This study evaluated the
incidence of genes responsible for carbapenemase production in carbapenem-resistant Pseudomonas aeruginosa and
assessed the genetic relatedness of the isolates by multi locus sequence typing (MLST).
Materials and Methods: Identification and antimicrobial susceptibility testing of P. aeruginosa isolates (n=234) by
the VITEK 2 system detected 81 carbapenem resistant P. aeruginosa isolates. PCR and DNA sequencing were used to
screen isolates for three metallo-β-lactamase encoding genes. MLST included amplification of seven housekeeping
genes and sequence type alignment using the online P. aeruginosa MLST database.
Results: Only the blaVIM-2 gene was detected in 15 of the 81 carbapenem resistant isolates. MLST indicated six
different novel sequence types among the blaVIM-2 positive P. aeruginosa isolates with the majority of the isolates
(9/15) containing identical allelic profiles of the sequence type allocated ST1 (provisionally assigned sequence type,
awaiting addition of new sequence types to PubMLST database). Five of these ST1 isolates were from patients and an
environmental sample in the same hospital ward suggesting an environmental reservoir. Carbapenem resistance in the
blaVIM-2 negative isolates may be due to other mechanisms.
Conclusion: The incidence of genes responsible for carbapenemase production in carbapenem-resistant Pseudomonas
aeruginosa and genetic relatedness of these isolates in public healthcare facilities within the Port Elizabeth area is of
concern and requires further investigation.
 
Publisher African Traditional Medicine Supporters Initiative (ATHMSI), 7, Road 1, Otunmaiye Square
 
Contributor Nelson Mandela Metropolitan University Research Themes grant for funding the study. Vanessa Pearce and the Bacteriology Laboratory staff at the National Health Laboratory Services, Port Elizabeth are acknowledged for their assistance with collection of cu
 
Date 2017-06-08
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://journals.sfu.ca/africanem/index.php/AJID/article/view/4793
10.21010/ajid.v11i2.9
 
Source African Journal of Infectious Diseases (AJID); Vol 11, No 2 (2017); 68-74
2505-0419
2006-0165
 
Language eng
 
Relation http://journals.sfu.ca/africanem/index.php/AJID/article/view/4793/2996
 
Coverage Africa


 
Rights Copyright (c) 2017 African Journal of Infectious Diseases (AJID)
 

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