Comparison between the Brix Refractometer and ELISA for Assessment of Colostrum IgG of Montbeliard x Holstein Cross Dairy Cows

Tropical Animal Science Journal

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Title Comparison between the Brix Refractometer and ELISA for Assessment of Colostrum IgG of Montbeliard x Holstein Cross Dairy Cows
Creator Fahim, N. H.
Imbabi, T. A.
Subject refractometer
Montbeliard x Holstein
Description The objectives of the present study were to examine the association between the brix refractometer% and ELISA assays in the assessment of colostrum-IgG content and in studying the factors affecting this content. Contents of IgG in colostrum samples of 132 healthy primiparous and multiparous Montbeliard x Holstein cross dairy cows kept in Egypt were evaluated using ELISA assay and brix refractometer%. The ELISA results showed that the IgG contents in the colostrum samples ranged from 5.96 to 114.84 mg/mL, with a mean of 57.07 mg/mL. Brix% results ranged from 15% to 35%, with a mean of 23.97%. The correlation and regression coefficients between ELISA(IgG) and brix% were significantly high (r=0.68 and r2 =0.59, respectively). Prediction regression equation was ELISA(IgG) = 31.81+1.12(brix refractometer%). The effects of parity and season of calving on both ELISA(IgG) and brix% were significant. ELISA(IgG) and brix% were higher in cows ≥ 3 lactations than those in the first and second lactations. Moreover, summer calving recorded higher ELISA(IgG) and brix% than autumn calving. Either ELISA(IgG) or brix% were not significantly affected by calf’s sex, single/twin births, or history of cows’ diseases. ELISA(IgG) and brix% had a significant positive correlation with calf’s birth weights and gestation lengths, while they recorded a non-significant correlation with dry-period length. It can be concluded that a refractometer is a simple on-farm tool to assess colostrum quality. Its high correlation with the lab analytical assay of ELISA confirms the validity of using refractometers by researchers in colostrum studies.
Publisher Faculty of Animal Science, Bogor Agricultural University
Date 2021-08-19
Type info:eu-repo/semantics/article
Peer-reviewed Article
Format application/pdf
Source Tropical Animal Science Journal; Vol. 44 No. 3 (2021): Tropical Animal Science Journal; 356-362
Language eng
Rights Copyright (c) 2021 Tropical Animal Science Journal

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