Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice

Sains Medika

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Title Stem Cell Mesenchymal Injection Increases Platelet-Derived Growth Factors Level and Percentage of Collagen in Third-Degree Burn injured Mice
 
Creator Ridawati, Erna
 
Subject Mesenchymal stem cells; PDGF; Collagen.
 
Description Introduction: Mesenchymal stem cell (MSC) has been demonstrated to accelerate wound healing. Platelet-derived growth factor (PDGF) and collagen have an important role in wound healing. However, the effect of MSC on levels of PDGF and number of collagen has not been established. Objectives: To determine the effect of MSC on the levels of PDGF and the percentage of collagen in the burns healing. Methods: in this experimental study, 20 BALB /c mice were divided into 4 groups: a control group (G-0) injected with 2 ml of distilled water and group 1 (G-1), Group 2 (G-2), and group 3 (G- 3) injected with MSC at the dose of 1 x 104, 2 x 104, and 4 x 104 respectively. The burn wound was made by attaching a metal nail that had been heated for 20 seconds at the feet of mice. Levels of PDGF on day 2 (PDGF-2) and 7 (PDGF-7) was assessed by ELISA, while on day 10 excision of skin was done to calculate the percentage of collagen. Results: There was a significant difference in the levels of PDGF-2, PDGF-7, and the percentage of collagen ( P <0.05). Posthoc analysis showed that the levels of PDGF-2, PDGF-7, and percentage of collagen in the G-2 and G-3 was significantly higher than that of G-0, p <0.05. While the levels of PDGF-2, PDGF-7, and the percentage of collagen in the G-3 was significantly higher than that of G-2 and G-1 (p <0.05). Conclusion: Injection of MSC at a dose of 2 x 1044 and 4 x 1044 increases the levels of PDGF and the percentage of collagen.
 
Publisher Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)
 
Contributor
 
Date 2017-03-09
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/1173
 
Source Sains Medika; Vol 7, No 2 (2016): July-December 2016; 49-53
2339-093X
2085-1545
 
Language eng
 
Relation http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/1173/906
 
Rights Copyright (c) 2017 Erna Ridawati
http://creativecommons.org/licenses/by/4.0
 

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