Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme

Tropical Animal Science Journal

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Field Value
 
Title Extraction of Collagen from the Skin of Kacang Goat and Production of Its Hydrolysate as an Inhibitor of Angiotensin Converting Enzyme
 
Creator Hakim, T. R.
Pratiwi, A.
Jamhari, Jamhari
Fitriyanto, N. A.
Rusman, Rusman
Abidin, M. Z.
Matulessy, D. N.
Erwanto, Y.
 
Subject antihypertensive
chymotrypsin
collagen of goat skin
hydrolysate
IC50
 
Description The study was designed to determine the potential of collagen hydrolysate produced from the skin of Kacang goat through chymotrypsin hydrolysis to be used as an inhibitor of angiotensin converting enzyme (ACE). This research was conducted in three replications, with the measured parameters include ACE inhibitory potential and collagen hydrolysate fractionation. The results showed that collagen extraction of Kacang goat skin by chymotrypsin hydrolysis yielded 9.74% (dry matter, v/v) collagen, with pH at 6.6. The extracted collagen contained α1, α2, and β collagen chains with molecular weights of 151 kDa, 141 kDa, and 240 kDa, respectively. Furthermore, the collagen hydrolysis produced protein peptides confirmed at molecular weights of 43 to 107 kDa. The hydrolysate fractionation at molecular weights of <3 kDa, 3-5 kDa, and >5 kDa showed proteins concentrations of 2.33 mg/mL, 3.81 mg/mL, and 3.93 mg/mL, respectively. The hydrolysate fractionation with molecular weight <3 kDa showed to have ACE inhibition activity with the IC50 value of 0.47 mg/mL. The study concluded that collagen hydrolysate extracted from the skin of Kacang goat had a promising potential as a source of antihypertensive agent.
 
Publisher Faculty of Animal Science, Bogor Agricultural University
 
Date 2021-06-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://journal.ipb.ac.id/index.php/tasj/article/view/32160
10.5398/tasj.2021.44.2.222
 
Source Tropical Animal Science Journal; Vol. 44 No. 2 (2021): Tropical Animal Science Journal; 222-228
2615-790X
2615-787X
10.5398/tasj.2021.44.2
 
Language eng
 
Relation http://journal.ipb.ac.id/index.php/tasj/article/view/32160/21704
 
Rights Copyright (c) 2021 Tropical Animal Science Journal
http://creativecommons.org/licenses/by-sa/4.0
 

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