The Effect of Green Tea and Stevia Extract on Liver Histopathology Wistar Rats Type II Diabetes Mellitus

Sains Medika

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Title The Effect of Green Tea and Stevia Extract on Liver Histopathology Wistar Rats Type II Diabetes Mellitus
THE EFFECT OF GREEN TEA AND STEVIA EXTRACT ON LIVER HISTOPATHOLOGY WISTAR RATs TYPE II DIABETES MELLITUS
 
Creator Novit, Elmawati
Sadhana, Udadi
Mahati, Endang
 
Subject green tea leaf; stevia leaf; liver; T2DM
green tea leaf;stevia leaf;liver;T2DM
 
Description Introduction: Glucose metabolism abnormality on type 2 diabetes mellitus (T2DM) caused liver damage due to dyslipidemia and increasing oxidative stress. Antidiabetic and antioxidants within green tea leaf and stevia leaf extract prevent liver damage due to T2DM complications. Objective: To investigate green tea and stevia leaf extract effects on liver histopathology on T2DM rats.Methods: This study used Post Test Only Control Group Design. A total of 30 Wistar rats were divided into 5 groups: control group (C-G), normal rats; negative control group (NC-G), T2DM induction; positive control group (PC-G), treated by Metformin; the first treatment group (T1-G), was treated by green tea leaf extract and stevia leaf extract; and the second treatment group (T2-G) was treated by a Metformin also green tea and stevia leaf extract. The treatment was given to subjects for 21 days starting 3 days after the induction of Nicotinamide (NA) and Streptozotocine (STZ). Histopathological assessment of liver cells uses assessment parameters in the form of features of necrosis, vacuolization, sinusoidal dilation and number of inflammatory cells. Hypothesis will be tested by Kruskal-Wallis (p<0,05) dan Mann-Whitney test (P<0,05) Results: The difference of liver damage in all group shows a significant difference, p<0.05. Significant differences in liver damage scores between the 2 groups there are between the negative control group and the control group (0.031), positive control (0.042), and second treatment group (0.011), and between first treatment group and second treatment group (0.041).Conclusion: The administration of green tea extract, stevia leaf, and standard drug may reduce liver damage score of Wistar with T2DM
ABSTRACTBackground: Glucose metabolism abnormality on T2DM caused liver damage due to dyslipidemia and increasing oxidative stress. Antidiabetic and antioxidants within green tea leaf and stevia leaf extract prevent liver damage due to T2DM complications. Objective: To know green tea and stevia leaf extract effects on liver histopathology on T2DM rats.Methods: This study was used Only Control Group Post-Test Design. A total of 30 Wistar rats were divided into 5 groups: control group, normal rats; negative control group, T2DM induction; positive control group, treated by the standard drug Metformin 150 mg/kg body weight; treatment group 1 was treated by green tea leaf extract 600 mg/kg body weight and stevia leaf extract 300 mg/kg body weight; and the treatment group 2 was treated by a standard drug also green tea and stevia leaf extract. Animal treatment started on 3 days after T2DM induction with NA+STZ and done for 21 days. Histopathological assessment of liver cells uses assessment parameters in the form of features of necrosis, vacuolization, sinusoidal dilation and number of inflammatory cells.Results: The difference of liver damage in the all group shows a significant difference with a value of p = 0.023. Significant differences in liver damage scores between the 2 groups there are between the negative control group and the control group (0.031), positive control (0.042), and treatment group 2 (0.011), and between training group 1 and treatment group 2 (0.041).Conclusion: The administration of green tea extract, stevia leaf, anda standard drug may reduce liver damage score of Wistar with T2DM
 
Publisher Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)
 
Contributor Diponegoro University
Diponegoro University
 
Date 2021-04-28
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/7437
10.30659/sainsmed.v12i1.7437
 
Source Sains Medika: Jurnal Kedokteran dan Kesehatan; Vol 12, No 1 (2021): January - June 2021; 7-13
2339-093X
2085-1545
10.30659/sainsmed.v12i1
 
Language eng
 
Relation http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/7437/pdf
 
Rights Copyright (c) 2021 Sains Medika: Jurnal Kedokteran dan Kesehatan
 

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