Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats

Sains Medika

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Title Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats
 
Creator Saputro, Arief Adi
Nasihun, Taufiqurrachman
Hussaana, Atina
 
Subject Propolis extract (CMCE method); TNF-α levels; CRP levels
 
Description Introduction: Carbon tetrachloride (CCl4) is a hepatotoxic substance inducing inflammation in liver cells. Inflammation is marked by increase in proinflammatory cytokines level such as tumor necrosis factor alpha (TNF-α), and C-reactive protein (CRP) produced by hepatocytes. Propolis extracted using continuous multi-stage countercurrent Extraction (CMCE propolis extract) contains active balsamic, caffeic acid phenetyl ester (CAPE), and flavonoids capable of affecting pro-inflammatory cytokines. Objective: to investigate the effect of CMCE propolis extract on TNF-α and CRP levels in CCl4-induced liver damage.Methods: In this study using a post test only control group design, 28 male Wistar rats were divided into four groups. The control group (C-G) was given CCl4 0.4 ml/200 g on day 14. The P3-G, P7-G, P14-G groups were treated with 3.6 mg/200 g, 7.2 mg/200 g, 14.4 mg/200 g CMCE propolis extract for 14 days and given CCl4 0.4 ml/g on day 14. The TNF-α and CRP levels were assessed using the ELISA kit one-way ANOVA and Post Hoc test (LSD) were applied for the statistical analysis. Results: TNF-α level in P14-G group (3.26 ± 0.93), P7-G (6.17+0.78), and P3-G (10.4±1.34) were significantly lower than that of C-G (17.29+1.56) (p<0.05). CRP levels in P14-G group (0.61±0.09), P7-G (1.38+0.48), and P3-G (12.01±0.65) were significantly lower than those of C-G (2.95+0.668) (p<0.05). The greatest reduction in TNF-α & CRP level was found in the group given propolis at a dose of 14.4 mg/200 g.Conclusion: The administration of CMCE propolis extract reduces TNF-α & CRP levels in CCl4-induced liver damage.
 
Publisher Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)
 
Contributor
 
Date 2021-04-26
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/5467
10.30659/sainsmed.v12i1.5467
 
Source Sains Medika: Jurnal Kedokteran dan Kesehatan; Vol 12, No 1 (2021): January - June 2021; 1-6
2339-093X
2085-1545
10.30659/sainsmed.v12i1
 
Language eng
 
Relation http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/5467/pdf
 
Rights Copyright (c) 2021 Sains Medika: Jurnal Kedokteran dan Kesehatan
 

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