The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis

Sains Medika

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Title The Administration of Topical Aloe vera Extract Reduce the Number of Sunburn Cells and Expression of Caspase-3 on Post UVB-light-exposure Epidermis
 
Creator Soetrisno, Kusumawati
Subchan, Prasetyowati
Hussana, Atina
 
Subject Ultraviolet B, Aloe Vera, sunburn cell, Caspase-3
 
Description Introduction: Ultraviolet B (UVB) radiation triggers the formation of free radicals that cause apoptosis and sunburn cells (SBC) formation. Aloe vera contains anti-inflammatory and antioxidant compounds that can potentially inhibit this process. Objective: to assess the effect of topical Aloe vera extract administration on the decrease of SBC number and Caspase-3 expression on the epidermis after UVB light exposure.Methods: In a post-test only group design study, a single dose of 3 doses of Erythema (DEM) was performed on two groups of 6 week old BALB/c female rats. Group A (control) did not receive any topical treatment, and group B (treatment) were smeared with 75% Aloe vera extract before irradiation. Each group was divided into 4 sub-groups based on post-exposure time of 6 hours (A1 and B1), 12 hours (A2 and B2), 24 hours (A3 and B3), and 48 hours (A4 and B4). The expression of caspase-3 was assessed by immunohistochemical staining while the SBC number was measured using a microscope. The expression of caspase-3 was analyzed using the Kruskal-Wallis and Mann-Whitney statistical tests, while the number of SBC was analyzed using the one-way ANOVA statistical test and the post-hoc LSD test.Results: The results of the Kruskal-Wallis analysis showed a significant difference in Caspase-3 expression between groups, p <0.05. The results of Mann-Whitney analysis showed a significant difference in Caspase-3 expression between the A1-A3 and B1-B3 subgroups (p <0.05). One-way ANOVA analysis showed a significant difference in the number of SBC between groups (p <0.05). Post-hoc LSD analysis showed significant differences in SBC counts between groups A and B across all subgroups, p <0.05.Conclusion: Topical application of Aloe Vera extract decreased Caspase-3 expression and the number of SBC in UVB light-exposed skin.
 
Publisher Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)
 
Contributor Universitas Islam Sultan Agung
Patologi Anatomi RS Sarjito Yogyakarta
 
Date 2021-01-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/9094
10.30659/sainsmed.v11i2.9094
 
Source Sains Medika: Jurnal Kedokteran dan Kesehatan; Vol 11, No 2 (2020): July-December 2020; 89-96
2339-093X
2085-1545
10.30659/sainsmed.v11i2
 
Language eng
 
Relation http://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/9094/pdf
 
Rights Copyright (c) 2021 Sains Medika: Jurnal Kedokteran dan Kesehatan
http://creativecommons.org/licenses/by/4.0
 

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