Rickettsia pada Pinjal Tikus (Xenopsylla Cheopis) di Daerah Pelabuhan Semarang, Kupang dan Maumere

Buletin Penelitian Kesehatan

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Title Rickettsia pada Pinjal Tikus (Xenopsylla Cheopis) di Daerah Pelabuhan Semarang, Kupang dan Maumere
 
Creator Joharina, Arum Sih; B2P2VRP Salatiga
Mulyono, Arief; Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Jl. Hasanudin No. 123 PO BOX 200 Salatiga, Indonesia
Sari, Tika Fiona; Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Jl. Hasanudin No. 123 PO BOX 200 Salatiga, Indonesia
Rahardianingtyas, Esti; Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Jl. Hasanudin No. 123 PO BOX 200 Salatiga, Indonesia
Wicaksonoputro, Dimas Bagus; Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Jl. Hasanudin No. 123 PO BOX 200 Salatiga, Indonesia
Pracoyo, Noer Endah; Pusat Penelitian dan Pengembangan Upaya Kesehatan Masyarakat Jl. Percetakan Negara 29 Jakarta 10560, Indonesia
Ristiyanto, Ristiyanto; Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Jl. Hasanudin No. 123 PO BOX 200 Salatiga, Indonesia
 
Subject Rickettsia spp., Xenopsylla cheopis, rat, molecular, Indonesia
 
Description Abstract The genus of Rickettsia is gram negative bacteria causing rickettsioses and involve mammal hosts and arthropod vectors in their life cycle (lices, mites, ticks, and fleas). Rats were one of rickettsial hosts, and fleas were rat ectoparasites that involve in the transmision from bacteria into humans. Unspecific clinical manifestation and difficulties of laboratory diagnoses caused the information about rickettsioses in humans were still limited. The aim of this study was to detect Rickettsia spp on rat fleas. Rat flea specimens were collected from three seaports of Semarang, Kupang and Maumere. Specimens were analyzed using PCR method by gltA amplification (primer 877F and 1258R). Confirmation of rickettsia species was conducted by sequencing. The results showed percentages of rickettsial infections on rat fleas for Semarang, Kupang, and Maumere were 19%, 61%, and 44%, respectively. Seven samples from eighteen samples sequences confirmed as Rickettsia typhi and the other 11 samples were Bartonella sp. This study was provided additional information about the presence of Rickettsia in 3 seaport in Indonesia and could be initiating rickettsioses surveilans in the regions. Keywords: Rickettsia spp., Xenopsylla cheopis, rat, molecular, IndonesiaAbstrakRickettsia merupakan bakteri pathogen penyebab berbagai rickettsiosis yang siklus hidupnya melibatkan reservoir mamalia dan vektor artropoda (kutu, tungau, caplak, dan pinjal). Tikus merupakan salah satu reservoir bakteri Rickettsia dan pinjal merupakan ektoparasit tikus yang berperan menularkan bakteri kepada manusia. Gejala klinis tidak spesifik dan diagnosis laboratorium yang sulit menyebabkan informasi mengenai rickettsiosis pada manusia masih sangat terbatas. Tujuan penelitian adalah mendeteksi Rickettsia spp. pada pinjal. Sampel pinjal tikus diambil dari tiga daerah pelabuhan yaitu Semarang, Kupang, dan Maumere. Sampel dianalisa menggunakan metode PCR dengan amplifikasi gen gltA (primer 877F dan 1258R). Sekuensing dilakukan untuk mengkonfirmasi spesies Rickettsia. Hasil penelitian menunjukkan bahwa persentase Rickettsia spp. pada pinjal tikus di Semarang 19%, Kupang 61%, dan Maumere 44%. Tujuh sampel dari 18 sampel yang disekuensing terkonfirmasi sebagai R.typhi dan 11 sampel sisanya merupakan Bartonella sp. Penelitian ini memberikan informasi tambahan tentang keberadaan Rickettsia di beberapa kota pelabuhan di Indonesia dan dapat dijadikan sebagai dasar surveilans rickettsiosis di Indonesia.Kata kunci: Rickettsia spp., Xenopsylla cheopis, tikus, molekuler, Indonesia
 
Publisher Badan Penelitian dan Pengembangan Kesehatan
 
Contributor Badan Litbang Kesehatan
 
Date 2016-12-30
 
Type
 
Format application/pdf
 
Identifier http://ejournal.litbang.kemkes.go.id/index.php/BPK/article/view/4920
10.22435/bpk.v44i4.4920.237-244
 
Source Buletin Penelitian Kesehatan; Vol 44, No 4 (2016); 237-244
Buletin Penelitian Kesehatan; Vol 44, No 4 (2016); 237-244
 
Language id
 
Rights The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
 

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