Efek N-Asetil-L- Sistein (NAC) terhadap Kadar Adma Plasma dan Ekspresi Protein Vcam-l pada Disfungsi Endotel Tikus Diabetes

Buletin Penelitian Kesehatan

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Title Efek N-Asetil-L- Sistein (NAC) terhadap Kadar Adma Plasma dan Ekspresi Protein Vcam-l pada Disfungsi Endotel Tikus Diabetes
 
Creator Santi, Irma; Program Magister Ilmu Farmasi, Fakultas Farmasi, Universitas Airlangga, Kampus B Unair JI. Dharmawangsa Dalam Surabaya 60286, lndonesia
Suprapti, Budi; Program Magister Ilmu Farmasi, Fakultas Farmasi, Universitas Airlangga, Kampus B Unair JI. Dharmawangsa Dalam Surabaya 60286, lndonesia
Susilo, Imam; Program Magister Ilmu Farmasi, Fakultas Farmasi, Universitas Airlangga, Kampus B Unair JI. Dharmawangsa Dalam Surabaya 60286, lndonesia
Khotib, Junaidi; Program Magister Ilmu Farmasi, Fakultas Farmasi, Universitas Airlangga, Kampus B Unair JI. Dharmawangsa Dalam Surabaya 60286, lndonesia
 
Subject (NAC), Endothelial dysfunction, ADMA, VCAM-1
 
Description Abstract Endothelial dysfunction is an early state of symptoms in a cardiovascular disease. An elevated oxidative stress plays a key role in the pathogenesis of macrovascular diabetic complication. The present study was design to evaluate the effect of NAC on plasma ADMA level and the expression of VCAM-1 protein on endothelial dysfunction in diabetic rats. Thirty male Sprague–Dawley rats were divided into 5 groups i.e. normal rats, diabetic rats, treatment with NAC 30 mg/kgBW, NAC 56 mg/ kgBW and NAC 100 mg/kgBW. Diabetic rats model was induced by intraperitonial administration of alloxan monohydrate at dose of 150 mg/kgBW, diabetes occurred on 3nd day after alloxan injection and then started treatment of N-acetyl-L-cystein for 28 days. ADMA plasma level was analyzed with Elisa Reader and the expression of VCAM-1 protein was evaluated by immunohistochemistry. Conclusion of this research is that treatment with NAC 30 mg/kgBW, NAC 56 mg/kgBW and NAC 100 mg/kgBW for 28 days may prevent oxidative stress indicated by the decreasing of plasma ADMA level by 45.8%: 55.75% and 65.92%, respectively and the decreasing of the expression of VCAM-1 protein on endothelial dysfunction in diabetic rat by 24%: 31.75% and 58.92%, respectively. Key words : (NAC), Endothelial dysfunction, ADMA, VCAM-1AbstrakDisfungsi endotel merupakan tahap awal pada penyakit kardiovaskular. Peningkatan stres oksidatif berperan penting dalam patogenesis komplikasi makrovaskular pada penyakit diabetes. Penelitian ini bertujuan untuk mengevaluasi efek N-asetil sistein (NAC) dalam menurunkan kadar ADMA plasma dan ekspresi protein VCAM-1 pada disfungsi endotel tikus diabetes. 30 tikus jantan strain sprague- Dawley dibagi menjadi 5 kelompok yaitu kelompok normal, kelompok diabetes, kelompok perlakuan NAC pada dosis 30, 56 dan 100 mg/kgBB. Model tikus diabetes diperoleh dengan diinduksi aloksan monohidrat 150 mg/kgBB secara intraperitonial, kondisi diabetes terjadi pada hari ke-3 setelah induksi aloksan selanjutnya dilakukan pemberian N-asetil sistein selama 28 hari. Kadar ADMA plasma dianalisis dengan metode ELISA dan ekspresi protein VCAM-1 dianalisis secara imunohistokimia. Kesimpulan dari penelitian ini bahwa pemberian NAC pada dosis 30, 56 dan 100 mg/kgBB selama 28 hari dapat mencegah peningkatan stres oksidatif yang ditandai dengan menurunnya kadar ADMA plasma berturut-turut 45,80%; 55,75% dan 65,92% serta menurunnya ekspresi dari protein VCAM-1 pada disfungsi endotel tikus diabetes berturut-turut 24,00% ;31,75% dan 58,92%.Kata kunci : ADMA, Endothelial dysfunction, NAC, VCAM-1
 
Publisher Badan Penelitian dan Pengembangan Kesehatan
 
Contributor
 
Date 2016-12-28
 
Type
 
Format application/pdf
 
Identifier http://ejournal.litbang.kemkes.go.id/index.php/BPK/article/view/4774
10.22435/bpk.v44i3.4774.147-152
 
Source Buletin Penelitian Kesehatan; Vol 44, No 3 (2016); 147-152
Buletin Penelitian Kesehatan; Vol 44, No 3 (2016); 147-152
 
Language en
 
Rights The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development) as publisher of the journal.Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development).Buletin Penelitian Kesehatan (Bulletin of Health Research) and Badan Penelitian dan Pengembangan Kesehatan (National Institute of Health Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.
 

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