Development of SSR loci in Prosopis tamarugo Phillipi and assessment of their transferability to species of the Strombocarpa section

Forest Systems

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Title Development of SSR loci in Prosopis tamarugo Phillipi and assessment of their transferability to species of the Strombocarpa section
 
Creator Contreras-Díaz, Roberto
S. Carevic, Felipe
Porcile, Vincenzo
Arias-Aburto, Mariana
 
Description Aims of the study: Phreatophyte species of the Prosopis genus are very important to natural ecosystems in Africa, South America and Asia due to their uses as food and seed sources and in agroforestry. In this research, through next-generation sequencing, we sought to search for and develop SSR markers in Prosopis tamarugo, in addition to assessing their transferability to other species in the Strombocarpa section.Area of study: The study was carried out in species of the Strombocarpa section collected in the “Pampa del Tamarugal”, located in the Atacama Desert (Chile); which is considered the driest and oldest desert on Earth.Materials and methods: The next-generation sequencing for the development of simple sequence repeat (SSR) or microsatellite loci for genetic research in P. tamarugo and their transferability in Prosopis burkartii and Prosopis strombulifera was used.Main results: A total of ~90.000 microsatellite loci in P. tamarugo were found, and a set of 43 primer pairs was used for validating SSR locus amplification. We found a large difference in the percentage of amplified SSR markers between species of the Strombocarpa and Algarobia sections.Research highlights: The present study provides for the first time 24 polymorphic SSR markers for species in the Strombocarpa section, which could be a useful tool for estimating genetic structure, developing breeding programs, quantifying genetic diversity and performing population studies.Keywords: Strombocarpa section; Prosopis tamarugo; Atacama Desert; microsatellites; NGS.
 
Publisher INIA
 
Contributor Universidad de Atacama
 
Date 2020-11-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://revistas.inia.es/index.php/fs/article/view/16706
10.5424/fs/2020292-16706
 
Source Forest Systems; Vol 29, No 2 (2020); e012
Forest Systems; Vol 29, No 2 (2020); e012
2171-9845
 
Language eng
 
Relation https://revistas.inia.es/index.php/fs/article/view/16706/4775
 
Rights info:eu-repo/semantics/openAccess
Copyright (c) 2020 Forest Systems
 

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