APPLICATION OF CRISPR ACTIVATION (CRISPRa) SYSTEM TO ZEBRAFISH (Danio rerio) GENES

Jurnal Mina Sains

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Field Value
 
Title APPLICATION OF CRISPR ACTIVATION (CRISPRa) SYSTEM TO ZEBRAFISH (Danio rerio) GENES
 
Creator Putri, Rizka Rahmana
 
Subject

 
Description CRISPR activation system is part of the function of CRSPR/Cas9 which is used to manipulate certain genes by activating these genes. CRISPR activation utilizes dCas9 (dead-Cas9) or Cas9 which is deactivated as DNA scissors, so that the use of Cas9 for gene activation does not cause targeted DNA chain termination. This research that uses this CRISPR activation is applied to the zebrafish gene (Danio rerio) aims to determine the mRNA level of the targeted gene. In this study, the ASCL1a and BCL6a genes from zebrafish were targeted as the object of study. The results showed that genes from zebrafish that were targeted had an increase in mRNA levels after being activated using CRISPR activation system. Keywords: CRISPR activation system (CRISPRa), zebrafish (Danio rerio), dCas9, ASCL1a gene, BCL6a gene
 
Publisher Universitas Djuanda
 
Contributor
 
Date 2019-12-31
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://ojs.unida.ac.id/jmss/article/view/2358
10.30997/jms.v5i2.2358
 
Source JURNAL MINA SAINS; Vol 5, No 2 (2019): Jurnal Mina Sains
2550-0759
2407-9030
10.30997/jms.v5i2
 
Language eng
 
Relation https://ojs.unida.ac.id/jmss/article/view/2358/pdf
 
Coverage


 
Rights Copyright (c) 2019 JURNAL MINA SAINS
 

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