Nanometer resolution imaging by single molecule switching

Nano Reviews & Experiments

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Title Nanometer resolution imaging by single molecule switching
Creator Hu, Dehong; Fundamental Science Directorate, Pacific Northwest National Laboratory
Orr, Galya
Description The fluorescence intensity of single molecules can change dramatically even under constant laser excitation. The phenomenon is frequently called as ‘blinking,’ and involves molecules switching between high- and low-intensity states (1). In addition to spontaneous blinking, the fluorescence of some special fluorophores, such as cyanine dyes and photoactivatable fluorescent proteins, can be switched on and off, by choice, using a second laser. Recent single-molecule spectroscopy investigations have shed light on mechanisms of single-molecule blinking and photoswitching. This ability to controllably switch single molecules led to the invention of a novel fluorescence microscopy with nanometer spatial resolution well beyond the diffraction limit.(Published: 2 April 2010)Citation: Nano Reviews 2010, 1: 5122 - DOI: 10.3402/nano.v1i0.5122
Publisher Co-Action Publishing
Contributor EMSL (A user facility of DOE-BER) and DOE-BES
Date 2010-04-02
Type info:eu-repo/semantics/article

Format application/pdf
Source Nano Reviews & Experiments; Vol 1 (2010) incl Supplements
Language eng

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