Evaluation of the magnetic field requirements for nanomagnetic gene transfection

Nano Reviews & Experiments

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Title Evaluation of the magnetic field requirements for nanomagnetic gene transfection
Creator Fouriki, A
Farrow, N
Clements, MA
Dobson, Jon; Guy Hilton Research Centre
Description The objective of this work was to examine the effects of magnet distance (and by proxy, field strength) on nanomagnetic transfection efficiency. Methods: Non-viral magnetic nanoparticle-based transfection was evaluated using both static and oscillating magnet arrays. Results: Fluorescence intensity (firefly luciferase) of transfected H292 cells showed no increase using a 96-well NdFeB magnet array when the magnets were 5 mm from the cell culture plate or nearer. At 6 mm and higher, fluorescence intensity decreased systematically. Conclusion: In all cases, fluorescence intensity was higher when using an oscillating array compared to a static array. For distances closer than 5 mm, the oscillating system also outperformed Lipofectamine 2000TM. Keywords: non-viral gene delivery; magnetic nanoparticle-based gene transfection; magnetic nanoparticles; magnetic field(Published: 9 July 2010)Citation: Nano Reviews 2010, 1: 5167 - DOI: 10.3402/nano.v1i0.5167
Publisher Co-Action Publishing
Date 2010-07-09
Type info:eu-repo/semantics/article

Format application/pdf
Identifier http://www.nanoreviewsexperiments.net/index.php/nano/article/view/5167
Source Nano Reviews & Experiments; Vol 1 (2010) incl Supplements
Language eng
Relation http://www.nanoreviewsexperiments.net/index.php/nano/article/view/5167/5773

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