Synthesis of protein-coated biocompatible methotrexate-loaded PLA-PEG-PLA nanoparticles for breast cancer treatment

Nano Reviews & Experiments

View Publication Info
 
 
Field Value
 
Title Synthesis of protein-coated biocompatible methotrexate-loaded PLA-PEG-PLA nanoparticles for breast cancer treatment
 
Creator Massadeh, Salam; King Abdulla International Medical Research Center (KAIMRC) Ministry of National Guard-Health affairs Kingdom of Saudi Arabia
Alaamery, Manal; King Abdulla International Medical Research Center (KAIMRC) Ministry of National Guard-Health affairs Kingdom of Saudi Arabia
Al-Qatanani, Shatha; King Abdulla International Medical Research Center (KAIMRC) Ministry of National Guard-Health affairs Kingdom of Saudi Arabia
Alarifi, Saqer; King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, College of Pharmacy, King Abdul Aziz Medical City, Ministry of National Guard Health Affairs, Riyadh, KSA
Bawazeer, Shahad; King Abdulla International Medical Research Center (KAIMRC) Ministry of National Guard-Health affairs Kingdom of Saudi Arabia
Alyafee, Yusra; King Abdulla International Medical Research Center (KAIMRC) Ministry of National Guard-Health affairs Kingdom of Saudi Arabia
 
Subject PEG; PLA; biodegradable nanoparticles; particle size; encapsulation efficiency; double emulsion method; poly(vinyl alcohol)
 
Description Background: PLA-PEG-PLA triblock polymer nanoparticles are promising tools for targeted dug delivery. The main aim in designing polymeric nanoparticles for drug delivery is achieving a controlled and targeted release of a specific drug at the therapeutically optimal rate and choosing a suitable preparation method to encapsulate the drug efficiently, which depends mainly on the nature of the drug (hydrophilic or hydrophobic). In this study, methotrexate (MTX)-loaded nanoparticles were prepared by the double emulsion method.Method: Biodegradable polymer polyethylene glycol-polylactide acid tri-block was used with poly(vinyl alcohol) as emulsifier. The resulting methotrexate polymer nanoparticles were coated with bovine serum albumin in order to improve their biocompatibility. This study focused on particle size distribution, zeta potential, encapsulation efficiency, loading capacity, and in vitro drug release at various concentrations of PVA (0.5%, 1%, 2%, and 3%).Results: Reduced particle size of methotrexate-loaded nanoparticles was obtained using lower PVA concentrations. Enhanced encapsulation efficiency and loading capacity was obtained using 1% PVA. FT-IR characterization was conducted for the void polymer nanoparticles and for drug-loaded nanoparticles with methotrexate, and the protein-coated nanoparticles in solid state showed the structure of the plain PEG-PLA and the drug-loaded nanoparticles with methotrexate. The methotrexate-loaded PLA-PEG-PLA nanoparticles have been studied in vitro; the drug release, drug loading, and yield are reported.Conclusion: The drug release profile was monitored over a period of 168 hours, and was free of burst effect before the protein coating. The results obtained from this work are promising; this work can be taken further to develop MTX based therapies.Keywords: PEG; PLA; biodegradable nanoparticles; particle size; encapsulation efficiency; double emulsion method; poly(vinyl alcohol)(Published: 16 June 2016)Citation: Nano Reviews 2016, 7: 31996 - http://dx.doi.org/10.3402/nano.v7.31996
 
Publisher Co-Action Publishing
 
Contributor KAIMRC
 
Date 2016-06-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
text/html
application/epub+zip
application/xml
 
Identifier http://www.nanoreviewsexperiments.net/index.php/nano/article/view/31996
10.3402/nano.v7.31996
 
Source Nano Reviews & Experiments; Vol 7 (2016) incl Supplements
2002-2727
 
Language eng
 
Relation info:eu-repo/grantAgreement/EC/FP7/001210
http://www.nanoreviewsexperiments.net/index.php/nano/article/view/31996/47199
http://www.nanoreviewsexperiments.net/index.php/nano/article/view/31996/47200
http://www.nanoreviewsexperiments.net/index.php/nano/article/view/31996/47201
http://www.nanoreviewsexperiments.net/index.php/nano/article/view/31996/47202
http://www.nanoreviewsexperiments.net/index.php/nano/article/downloadSuppFile/31996/27794
 
Rights info:eu-repo/semantics/openAccess
Copyright (c) 2016 Salam Massadeh
http://creativecommons.org/licenses/by-nc/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library