Detection of HBV DNA by PCR and its application in clinical transfusion
Reports in Clinical Studies and Medicine
View Publication Info| Field | Value | |
| Title |
Detection of HBV DNA by PCR and its application in clinical transfusion
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| Creator |
Li, Shunqing
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| Subject |
Hepatitis B virus, HBsAg, serological markers, screening
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| Description |
This study was to detect the hepatitis B virus (HBV) DNA copies in patients through blood transfusions; recessive carriers with HBsAg negative but HBV DNA positive were further studied to see the content and distribution of HBV in patients, and provide evidence for the clinical treatment. A total of 532 blood samples collected from July 2014 to July 2015 were tested for HBV-DNA viral load and hepatitis B serological markers using quantitative Polymerase Chain Reaction (qPCR) and serologic test (five serological markers of hepatitis B). The results showed that, 3 cases were HBV serology negative and the HBV-DNA viral load was in the range of 250-500 whereas only 1 case was HBsAb positive and the HBV-DNA viral load was above 500. qPCR, for detecting HBV DNA, together with serological routine test can effectively reduce HBV infection during transfusion and prevent medical disputes.
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| Publisher |
PiscoMed Publishing Pte Ltd
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| Contributor |
—
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| Date |
2018-12-27
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| Type |
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion — |
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| Format |
application/pdf
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| Identifier |
http://ojs.piscomed.com/index.php/rcsm/article/view/13
10.18282/rcsm.v1.i1.13 |
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| Source |
Reports in Clinical Studies and Medicine; Vol 1, No 1 (2019): Published; 17-20
2661-4006 |
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| Language |
eng
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| Relation |
http://ojs.piscomed.com/index.php/rcsm/article/view/13/32
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| Rights |
Copyright (c) 2019 Li Shuqing
http://creativecommons.org/licenses/by-nc/4.0 |
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