TEKHNIK PEWARNAAN NEURON DAN NEUROGLIA PADA SISTEM SARAF PUSAT

Majalah Biomorfologi

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Field Value
 
Title TEKHNIK PEWARNAAN NEURON DAN NEUROGLIA PADA SISTEM SARAF PUSAT
 
Creator Rimbun, Rimbun
Kalanjati, Viskasari Pintoko
 
Subject Nervous system; histological preparation; staining method; Sistem saraf; preparat histologi; metode pewarnaan
 
Description Neurological disorders become a significant and growing problems. Both clinical and preclinical researchers conducted experiments in human tissue and animal model. The preparation of animal’s tissue needs the right staining methode to show the wanted cells or structures clearly. The nervous tissue contains neurons and neuroglial cells (astrocytes, oligodendrocytes, microglia, and ependyma). The routine staining such as Haematoxyllin and Eosin, more clearly shows the nucleus and organells within the cytoplasm of neurons and neuroglias, but less clearly shows their specific morphology. The heavy metal impregnation techniques, such as silver staining, gold staining, Golgi method, Cajal method, and Rio Hortega method, can overcome this problem and clearly show the morphology of neuron and neuroglia also the neuroanatomical connections in the brain. Other alternative stainings for nervous tissue are Nissl staining, tolluidin blue, methylene blue and Luxol fast blue. There are also immunohystochemical staining methods, i.e. antibody against glial fibrillary acidic protein (GFAP) for astrocyte, monoclonal antibody againts human leucocyte antigen (HLA) for microglia, etc. Based on the variation of staining methods in the nervous tissue, researchers must have a good plan to carefully determine which staining method should be used.
 
Publisher Department of Anatomy and Histology, Faculty of Medicine, Universitas Airlangga
 
Contributor
 
Date 2019-11-15
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier https://e-journal.unair.ac.id/MBIO/article/view/15910
10.20473/mbiom.v25i2.2012.33-40
 
Source Majalah Biomorfologi; Vol 25, No 2 (2012): Majalah Biomorfologi; 33-40
2716-0920
0215-8833
 
Language eng
 
Relation https://e-journal.unair.ac.id/MBIO/article/view/15910/8578
 
Rights Copyright (c) 2019 Majalah Biomorfologi
http://creativecommons.org/licenses/by-nc-sa/4.0
 

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