Identification of canine parvovirus type 2C in puppies of Nicaragua

Revista MVZ Córdoba

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Title Identification of canine parvovirus type 2C in puppies of Nicaragua
Identificación de parvovirus canino tipo 2C en cachorros de Nicaragua
 
Creator Flores-Somarriba, Byron
Saénz, Jairo
Gutiérrez-Soza, Jorge
Sheleby-Elías, Jessica
Fuertes-Negro, Héctor
Halihel-Kassab, Nabil
 
Description Objective. To identify genotypes of canine parvovirus circulating in puppies in two municipalities of Nicaragua. Materials and methods. Rectal swab samples were collected from 45 puppies (less than 6 months of age) with or without a vaccination history, showing or not symptomatology compatible with parvoviruses. The samples and two of the vaccines that are marketed in Nicaragua (vaccine nº1 and vaccine nº2) were analyzed by conventional Polymerase Chain Reaction (PCR) to a product of ≈ 630 bp of the VP2 gene. In addition, four randomly chosen field samples and both vaccine strains were sequenced in reverse sense. Results. 28.9% (13/45) of the analyzed samples were positive by PCR, for the fragment of CPV VP2 gene. No significative difference (p≥0.05) was seen in PCR detection between dogs with or without vaccination history. The four sequenced field samples were identified as CPV-2C genotype while both vaccine strains were identified as CPV-2A genotype. Conclusions. The aligned sequences showed high evolutionary divergence of filed strains with respect to vaccines strains, leading us to rethink the efficacy of the analyzed vaccines which are nowadays commercially available in Nicaragua.
Objetivo. Identificar los genotipos de parvovirus canino-circulantes en cachorros en dos municipios de Nicaragua. Materiales y métodos. Se recolectaron muestras por hisopado rectal de 45 cachorros con y sin antecedentes de vacunación, con menos 6 meses de edad, con y sin sintomatología compatible con parvovirosis. Las muestras y dos de las vacunas que se comercializan en Nicaragua (vacuna nº1 y vacuna nº2) fueron analizadas por Reacción en Cadena de la Polimerasa (PCR) convencional para un producto de ≈ 630 pb del gen VP2. Además, se secuenciaron en sentido inverso cuatro muestras de campo elegidas al azar y ambas cepas de vacunas. Resultados. El 28.9% (13/45) de las muestras analizadas fueron positivas en PCR. No se encontraron diferencias significativas en la detección por PCR del fragmento de VP2, respecto al estado de vacunación de los animales (p≥0.05). Las cuatro muestras de campo secuenciadas fueron identificadas como genotipo CPV-2C y las dos cepas vacunales se identificaron como genotipo CPV-2A. Conclusiones. La inferencia evolutiva de las secuencias alineadas de cepas vacunales mostró alta divergencia evolutiva respecto a las cepas de campo. Este hallazgo lleva a replantear el tema sobre la eficacia de las vacunas analizadas en este trabajo y que son aplicadas en Nicaragua.
 
Publisher Universidad de Córdoba
 
Date 2020-04-20
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/pdf
application/zip
application/zip
application/xml
application/xml
 
Identifier https://revistamvz.unicordoba.edu.co/article/view/e1788
10.21897/rmvz.1788
 
Source Journal MVZ Cordoba; Journal MVZ Córdoba Volume 25(2) May August 2020; e1788
Revista MVZ Córdoba; Revista MVZ Córdoba Volumen 25(2) Mayo-Agosto 2020; e1788
1909-0544
10.21897/rmvz.v25.n2.2020
 
Language spa
eng
 
Relation https://revistamvz.unicordoba.edu.co/article/view/e1788/2458
https://revistamvz.unicordoba.edu.co/article/view/e1788/2459
https://revistamvz.unicordoba.edu.co/article/view/e1788/2466
https://revistamvz.unicordoba.edu.co/article/view/e1788/2467
https://revistamvz.unicordoba.edu.co/article/view/e1788/2468
https://revistamvz.unicordoba.edu.co/article/view/e1788/2469
 

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