Androgen derivatives affect immunopresence but not activity of antioxidant enzymes in pancreatic islets during diabetogen-induced apoptosis

Open Science Journal

View Publication Info
 
 
Field Value
 
Title Androgen derivatives affect immunopresence but not activity of antioxidant enzymes in pancreatic islets during diabetogen-induced apoptosis
 
Creator Palomar-Morales, Martin
 
Subject Diabetes mellitus; Involves Oxidative Stress; Androgens
Pancreatic islets, scavenging enzymes, androgen derivatives, apoptosis, immunohistochemistry
 
Description The onset of diabetes mellitus involves oxidative stress. Previous reports from our laboratory have shown that androgens, but not estrogens or progestogens, could prevent the apparition of apoptosis in beta-cell induced by streptozotocin in early stages of diabetes induction in rat; these effect was related with changes in antioxidant enzymes presence. The aim of this study was to evaluate the participation of scavenging enzymes in beta-cells protection under androgen derivatives, and their cytoprotective mechanism. Adult male rats were orchidectomyzed, and treated with androgens after STZ administration. Pancreatic catalase and superoxide dismutase-1 and -2 were analyzed by immunohistochemistry and TUNEL assay. In another experimental focusing, pancreatic islets were obtained from intact male rats, and incubated with alloxan in order to induce ROS production, in the presence of androgen derivatives, and the activities of catalase, superoxide dismutase and glutathione peroxidase were measured in cell lysates. Androgen treatment affects immunopresence of the antioxidant enzymes in pancreas, but not their activity in islets, suggesting that scavenging enzymes could be affected during beta-cells apoptosis.
 
Publisher Open Science Journal
 
Contributor This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
 
Date 2020-04-03
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier https://osjournal.org/ojs/index.php/OSJ/article/view/2256
10.23954/osj.v5i1.2256
 
Source Open Science Journal; Vol 5, No 1 (2020): Open Science Journal
2466-4308
 
Language eng
 
Relation https://osjournal.org/ojs/index.php/OSJ/article/view/2256/261
 
Rights Copyright (c) 2020 Open Science Journal
http://creativecommons.org/licenses/by/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library