DETERMINATION OF CELL TYPE AND HAEMOCYTE MORPHOMETRIC CHARACTERISTICS OF WESTERN AUSTRALIA FRESHWATER CRAYFISH (Cherax cainii) AT DIFFERENT TEMPERATURES IN VITRO

e-Jurnal Rekayasa dan Teknologi Budidaya Perairan

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Title DETERMINATION OF CELL TYPE AND HAEMOCYTE MORPHOMETRIC CHARACTERISTICS OF WESTERN AUSTRALIA FRESHWATER CRAYFISH (Cherax cainii) AT DIFFERENT TEMPERATURES IN VITRO
 
Creator Prastowo, Bambang Widyo
Lareu, Ricky
Caccetta, Rima
Fotedar, Ravi
 
Description The purpose of this study was to identify and morphologically characterize the hemocyte cell types of freshwater crayfish, Cherax cainii. In addition to morphological observations using a light microscope (LM) and electron transmission microscope (TEM), a flow cytometer (FCM) is also used. Three main types of haemocyte of C. cainii were identified by LM, TEM, and FCM. Determination of haemocyte by LM based on the number, size of cytoplasmic granules and the ratio of N:C. These cells are Hyaline (HC), Small Granule (SGC), and Large Granule (LGC) cells. Three types of haemocyte were also observed by TEM based on cell and nucleus size, granule diameter, number of cytoplasmic granules per cell and N:C. Haemocyte population was successfully detected with FCM based on forward scatter (FSC) signals, versus side scattering signals/side scatter (SSC), with plot data via scatter parameter gating. Three cluster formations were observed, which were temporarily classified as SGC, LGC, and HC regions. Morphometric analysis was performed with TEM on C. cainii haemocyte to measure various cellular features. Some morphological features vary between types of haemocyte and are also affected by temperature. Total hemocyte count (THC) and differential hemocyte count (DHC) are calculated using FCM. THC increases with higher temperatures, from 1,9 x 106 /ml at 20 °C to 4.9 x 106 /ml at 30 °C.  The most abundant hemocyte at all temperatures is HC, followed by SGC and LGC.
 
Publisher University of Lampung
 
Contributor
 
Date 2020-02-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://jurnal.fp.unila.ac.id/index.php/bdpi/article/view/3735
10.23960/jrtbp.v8i2.p1009-1028
 
Source e-Jurnal Rekayasa dan Teknologi Budidaya Perairan; Vol 8, No 2 (2020); 1009-1028
2597-5315
2302-3600
 
Language eng
 
Relation http://jurnal.fp.unila.ac.id/index.php/bdpi/article/view/3735/2754
 
Rights Copyright (c) 2020 Bambang Widyo Prastowo, Ricky Lareu, Rima Caccetta, Ravi Fotedar
http://creativecommons.org/licenses/by-sa/4.0
 

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