Administration of Combined Methanolic Leaf Extracts of Vernonia amygdalina and Gongronema latifolium Enhanced Glut 2 Expression in the Pancreas and Downregulates Serum Caspase 3 Activity of Streptozotocin-Induced Diabetic Wistar Rats

Journal of Advances in Medicine and Medical Research

View Publication Info
 
 
Field Value
 
Title Administration of Combined Methanolic Leaf Extracts of Vernonia amygdalina and Gongronema latifolium Enhanced Glut 2 Expression in the Pancreas and Downregulates Serum Caspase 3 Activity of Streptozotocin-Induced Diabetic Wistar Rats
 
Creator Akpaso, M. I.
Orie, N. N.
Ebong, P. E.
 
Description Aim: The study evaluated the effects of the combined extracts of Vernonia amygdalina (VA) and Gongronema latifolium (GL) on pancreatic GLUT 2 expression and caspase 3 activity in streptozotocin (STZ, 45 mg/Kg)-induced diabetic rats.
Study Design: Fifteen Albino rats were used for the study and were placed in 3 groups of 5 rats each: A - normal control, B – Diabetic control and C – experimental group.
Place and Duration of Study: The study was carried out in the department of Anatomy, University of Calabar.
Duration:  6 months.
Methodology:  Half of the diabetic rats were treated with VA+GL (400mg/kg, ratio 1:1, DE group) for 28 days, while the other half was untreated and served as diabetic control (DC). Normal control (NC) rats were untreated. After 28 days, the rats were sacrificed and their blood glucose, serum GLUT 2 and caspase 3 activity were measured.  Histochemical evaluation of the pancreas was also carried out.
Results: Blood glucose concentrations for the 3 groups were 60.31±7.28, 257.00±4.43, and 116.60±10.11 mg/dl for NC, DC and DE respectively. This represented a 4-fold increase in the DC compared with NC and a significant amelioration in the extract-treated DE group compared with DC group. Serum GLUT 2 concentrations were 70 ng/ml in NC, dropped to 8 ng/ml (p<0.05) in the DC and recovered to 20 ng/ml in DE (p<0.05). Serum caspase was 3.2 ng/ml for NC, increased to 8.5 ng/ml in DC (p<0.05) and reduced to 1.8 ng/ml in DE (p<0.05). The histology of the pancreas showed distorted, degenerated and shrunken β-cells mass in DC compared with NC and DE groups. The DE group showed clear signs of regeneration of the islet cells which was corroborated by positive Feulgen’s reaction compared with the DC group.
Conclusion: The data suggests that the combined VA+GL extract has the potential to effectively reverse pancreatic damage in diabetes.
 
Publisher SCIENCEDOMAIN international
 
Date 2019-10-04
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier http://journaljammr.com/index.php/JAMMR/article/view/30230
10.9734/jammr/2019/v30i930230
 
Source Journal of Advances in Medicine and Medical Research; 2019 - Volume 30 [Issue 9]
2456-8899
 
Language eng
 
Relation http://journaljammr.com/index.php/JAMMR/article/view/30230/56714
http://journaljammr.com/index.php/JAMMR/article/view/30230/56715
 
Rights Copyright (c) 2019 © 2019 Akpaso et al.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library