Acetylcholinesterase activity and total antioxidant levels in dogs with mammary tumors before and after surgical removal

Revista MVZ Córdoba

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Title Acetylcholinesterase activity and total antioxidant levels in dogs with mammary tumors before and after surgical removal
Actividad de acetilcolinesterasa y niveles totales de antioxidantes en perros con tumores de mama antes y después de la extirpación quirúrgica
 
Creator Faria, Joice LM
Rossi, Raissa I
Da Silva, Aleksandro S
Bottari, Nathieli B
Olsson, Débora
Dalberto, Jéssica L
Mendes, Ricardo E
Sueiro, Felipe AR
Pereira, Wanderson AB
Mueller, Eduardo N
Shetinger, Maria RC
Guarda, Naiara
Moresco, Rafael N
Baldissera, Matheus D
Machado, Gustavo
 
Description Objective. The aim of this study was to evaluate AChE activity in total blood and the FRAP levels in samples from dogs with mammary tumors before and after surgery, as well as the relationship between these variables with immunohistochemical markers of tumor (E-caderina, ki-67, COX-2). Materials and methods. In this study, 13 dogs with mammary tumors were divided into two groups (A and B). The group A was formed by dogs with tumors smaller than 3 cm of diameter, and the group B was formed by dogs with tumor of 3 cm of diameter or larger. The AChE activity and FRAP levels were evaluated before and after surgery and the immunohistochemistry were performed at the tumors. Results. The AChE activity was significantly increased (p<0.05) in dogs with mammary cancer compared to control animals, and neither surgery or tumor size affected the AChE activity (p>0.05). FRAP levels before surgery were significantly lower (p<0.05) compared to control animals. Also, FRAP levels increased significantly after surgery in animals of the group A compared to data before surgery, a fact not observed in dogs from the group B. E-cadherin showed low significant positive correlation with FRAP levels (r=0.37, P-value=0.05); COX-2 showed a moderate significant positive correlation to FRAP (r=0.55, P-value<0.05); and COX-2 showed a low significant positive correlation to AChE (r=0.32, P-value=0.01). Conclusions. AChE and antioxidant levels are modified in dogs with mammary cancer. These variables are involved in various physiological functions, and thus, they might be related to disease pathogenesis.
Objetivo. El objetivo de este estudio fue evaluar la actividad de la AChE en sangre total y los niveles de FRAP en muestras de perros con tumores mamarios antes y después de la cirugía, así como la relación entre estas variables con marcadores inmunohistoquímicos de tumores (E-caderina, ki-67 , COX - 2). Materiales y métodos. En este estudio, 13 perros con tumores mamarios se dividieron en dos grupos (A y B). El grupo A estaba formado por perros con tumores menores de 3 cm de diámetro y el grupo B estaba formado por perros con tumor de 3 cm de diámetro o más. La actividad de AChE y los niveles de FRAP se evaluaron antes y después de la cirugía y la inmunohistoquímica se realizó en los tumores. Resultados. La actividad de la AChE aumentó significativamente (p <0,05) en perros con cáncer mamario en comparación con los animales control, y ni la cirugía ni el tamaño tumoral afectaron la actividad de la AChE (p> 0,05). Los niveles de FRAP antes de la cirugía fueron significativamente más bajos (p <0,05) en comparación con los animales control. Además, los niveles de FRAP aumentaron significativamente después de la cirugía en animales del grupo A en comparación con los datos antes de la cirugía, hecho que no se observó en perros del grupo B. La E-cadherina mostró correlación positiva baja con los niveles de FRAP (r = 0,37, valor P = 0,05); COX-2 mostró una moderada correlación positiva significativa con FRAP (r = 0,55, P-valor <0,05); Y la COX-2 mostró una correlación positiva de baja significación con la AChE (r = 0.32, P-valor = 0.01). Conclusiones. AChE y los niveles de antioxidantes se modifican en perros con cáncer de mama. Estas variables están implicadas en diversas funciones fisiológicas, y por lo tanto, pueden estar relacionadas con la patogénesis de la enfermedad.
 
Publisher Universidad de Córdoba
 
Date 2018-09-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
application/epub+zip
application/xml
 
Identifier https://revistamvz.unicordoba.edu.co/article/view/1369
10.21897/rmvz.1369
 
Source Journal MVZ Cordoba; Revista MVZ Córdoba Volumen 23(3) Septiembre-Diciembre 2018; 6799-6812
Revista MVZ Córdoba; Revista MVZ Córdoba Volumen 23(3) Septiembre-Diciembre 2018; 6799-6812
1909-0544
 
Language spa
 
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https://revistamvz.unicordoba.edu.co/article/view/1369/epub
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