Activación y determinación de parámetros cinéticos de la Plasmina Humana y Ovis aries

Revista MVZ Córdoba

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Field Value
 
Title Activación y determinación de parámetros cinéticos de la Plasmina Humana y Ovis aries
 
Creator Cañas, Omaira
Quijano, Alfonso
Arbeláez, Fernando
 
Description Objetivo. Comparar las cinéticas y activaciones, unificar las purificaciones y determinar las secuencias de los terminales-N de los plasminógenos Ovis aries y humano. Materiales y métodos. Los plasminógenos fueron purificados por el mismo método: cromatografías de afinidad e intercambio iónico, activados con urocinasa, la secuencia de los terminales-N se realizó por el método de Edman Resultados. La afinidad de la plasmina Ovis aries por el sustrato cromogénico fue de 0.45 mM, 11.8 veces mayor que la afinidad de la plasmina humana (5.3 mM). Conclusiones. Se confirma y unifica el método de purificación de los plasminógenos del plasma, para todos los mamíferos. La alta afinidad de la plasmina Ovis aries confirma una mayor afinidad de las plasminas animales por el sustrato cromogénico, en comparación con la plasmina humana
 
Publisher Universidad de Córdoba
 
Date 2011-01-01
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://revistamvz.unicordoba.edu.co/article/view/295
10.21897/rmvz.295
 
Source Journal MVZ Cordoba; Revista MVZ Córdoba Volumen 16(1) Enero-Abril 2011
Revista MVZ Córdoba; Revista MVZ Córdoba Volumen 16(1) Enero-Abril 2011
1909-0544
 
Language spa
 
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https://revistamvz.unicordoba.edu.co/article/view/295/363
 

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