Mycelial inhibition of Trichoderma spp. isolated from the cultivation of Pleurotus ostreatus with an extract of Pycnoporus sp.

Acta Botanica Mexicana

View Publication Info
 
 
Field Value
 
Title Mycelial inhibition of Trichoderma spp. isolated from the cultivation of Pleurotus ostreatus with an extract of Pycnoporus sp.
Mycelial inhibition of Trichoderma spp. isolated from the cultivation of Pleurotus ostreatus with an extract of Pycnoporus sp.
 
Creator Talavera-Ortiz, Anaid
Chaverri, Priscila
Diaz-Godinez, Gerardo
Acosta-Urdapilleta, Ma de Lourdes
Villegas, Elba
Tellez-Tellez, Maura
 
Subject Disease control; edible mushrooms, lignocellulosic substrate
Disease control; edible mushrooms, lignocellulosic substrate
 
Description Abstract:Background and Aims: The production of edible fungi is affected by bacterial, fungal and viral diseases, which very often cause large losses. In the productionof mushrooms of the genus Pleurotus, the fungi of Trichoderma spp. represent a serious problem of contamination and although there are some chemical compoundsthat control the infection, they are not entirely safe for human consumption. As a consequence, alternatives are being searched for through biotechnology,such as the one presented in this paper.Methods: Strains of fungi of the genus Trichoderma were isolated from the substrate where Pleurotus ostreatus was being cultivated. These were identifiedmorphologically and molecularly, followed by tests to inhibit the growth of Trichoderma strains in both agar and wheat straw, using a cetonic extract of thedehydrated fruiting body of Pycnoporus sp.Key results: Two strains of Trichoderma (T. pleuroti and T. atrobrunneum, belonging to the clade of T. harzianum) were isolated from infected substrate obtainedin production modules of Pleurotus ostreatus located in Tlaquitenango and Cuernavaca, in the state of Morelos, Mexico. The effect of a cetonic extract of thefruiting body of Pycnoporus sp. on the mycelial growth of the isolated strains of Trichoderma was also evaluated, observing decrease in mycelial growth rate inPetri dish up to 72% and on lignocellulosic substrate both mycelial growth and sporulation were delayed up to 10 days.Conclusions: The extract of Pycnoporus sp. could be an alternative to control the infection by Trichoderma spp. in mushroom cultures of the genus Pleurotus.Key words: disease control, edible mushrooms, lignocellulosic substrate. 
Background and Aims: The production of edible fungi is affected by bacterial, fungal and viral diseases, which very often produces large losses. In the production of mushrooms of the genus Pleurotus, the fungi of the genus Trichoderma represent a serious problem of contamination and although there are some chemical compounds that control the infection, they are not entirely safe for human consumption, so they are looking for alternatives through biotechnology, such as the one presented in this paper. Methods: Strains of fungi of the genus Trichoderma were isolated from the substrate where Pleurotus ostreatus was being cultivated (characteristic contamination of green mold), they were identified morphologically and molecularly, later tests were carried out to inhibit the growth of Trichoderma strains in both agar and wheat straw using a cetonic extract of the fruiting body dehydrated of Pycnoporus sp. Key results: Two strains of Trichoderma (Trichoderma pleuroti and Trichoderma atrobrunneum, belonging to the clade of Trichoderma harzianum) were isolated from infected substrate obtained in production modules of Pleurotus ostreatus located in Tlaquitenango and Cuernavaca from the Morelos state, Mexico. On the other hand, the effect of a cetonic extract of the fruiting body of Pycnoporus sp. on the mycelial growth of the isolated strains of Trichoderma was evaluated, observing decrease in mycelial growth rate in Petri dish up to 72% and on lignocellulosic substrate both mycelial growth and sporulation were delayed up to 10 days. Conclusions: It is suggested that the extract of Pycnoporus sp. is an alternative to control the growth and sporulation of strains of Trichoderma in the cultivation of mushrooms of the genus Pleurotus.
 
Publisher Instituto de Ecología, A.C.
 
Contributor CREGENHC
CONACYT
CREGENHC
CONACYT
 
Date 2020-01-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

artículo evaluado por pares
 
Format application/pdf
application/xml
application/xml
application/epub+zip
 
Identifier http://abm.ojs.inecol.mx/index.php/abm/article/view/1537
10.21829/abm127.2020.1537
 
Source Acta Botanica Mexicana; Núm. 127 (2020)
Acta Botanica Mexicana; Núm. 127 (2020)
2448-7589
0187-7151
 
Language eng
 
Relation http://abm.ojs.inecol.mx/index.php/abm/article/view/1537/pdf
http://abm.ojs.inecol.mx/index.php/abm/article/view/1537/1951
http://abm.ojs.inecol.mx/index.php/abm/article/view/1537/1952
http://abm.ojs.inecol.mx/index.php/abm/article/view/1537/epub
http://abm.ojs.inecol.mx/index.php/abm/article/downloadSuppFile/1537/1588
http://abm.ojs.inecol.mx/index.php/abm/article/downloadSuppFile/1537/1589
http://abm.ojs.inecol.mx/index.php/abm/article/downloadSuppFile/1537/1590
http://abm.ojs.inecol.mx/index.php/abm/article/downloadSuppFile/1537/1591
 
Rights Copyright (c) 2019 Acta Botanica Mexicana
https://creativecommons.org/licenses/by-nc/4.0
 

Contact Us

The PKP Index is an initiative of the Public Knowledge Project.

For PKP Publishing Services please use the PKP|PS contact form.

For support with PKP software we encourage users to consult our wiki for documentation and search our support forums.

For any other correspondence feel free to contact us using the PKP contact form.

Find Us

Twitter

Copyright © 2015-2018 Simon Fraser University Library