TEKNIK PCR ITS-RFLP UNTUK SELEKSI ISOLAT JAMUR PADA PENGUJIAN AGEN PENGENDALI HAYATI PADA SERANGAN GANODERMA TANAMAN Acacia mangium

Jurnal Pemuliaan Tanaman Hutan

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Title TEKNIK PCR ITS-RFLP UNTUK SELEKSI ISOLAT JAMUR PADA PENGUJIAN AGEN PENGENDALI HAYATI PADA SERANGAN GANODERMA TANAMAN Acacia mangium
 
Creator Prihatini, Istiana
Faradilla, Farah Aulya
Suranto, Suranto
 
Subject fungal identification; isolates selection; restriction enzyme
 
Description In vivo control activities test of BCA candidate is required to evaluate its effectiveness. This test involved identification of the fungal species that inhabited the experimental plant after inoculation of BCA and pathogen. Identification of inhabitant fungi in the BCA control activity test can be conducted by culturing the fungi on artificial media. Fungal species can be identified based on morphological characters or genetic characters of fungal culture. This study was conducted to determine the potential of PCR ITS-RFLP molecular markers in the initial selection process of fungal isolates before identification based on DNA sequences and to select the enzyme that produce polymorphic PCR ITS RFLP pattern. Three enzymes (DraI, EcoRI and HinfI) were successfully separated 8 fungal cultures used in this study into three groups based on the pattern of PCR ITS DNA, while five other enzymes (BamHI, BclI HaeII, HpaI, and HindIII) were failed to cut the DNA ITS fragments, except for one isolate.
 
Publisher Center for Forest Biotechnology and Tree Improvement (CFBTI)
 
Contributor
 
Date 2019-06-30
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion

 
Format application/pdf
 
Identifier http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5215
10.20886/jpth.2019.13.1.%p
 
Source Jurnal Pemuliaan Tanaman Hutan; Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan
2527-8665
1693-7147
 
Language eng
 
Relation http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPTH/article/view/5215/4742
 
Rights Copyright (c) 2019 Jurnal Pemuliaan Tanaman Hutan
https://creativecommons.org/licenses/by-nc-sa/4.0
 

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